Reactome | RPA complexed to ssDNA [nucleoplasm]

RPA complexed to ssDNA [nucleoplasm]

Stable Identifier

R-HSA-176293

Type

Complex

Species

Homo sapiens

Compartment

nucleoplasm

Synonyms

RPA-ssDNA complex

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General

RPA associates with ssDNA in distinct complexes that can be distinguished by the length of ssDNA occluded by each RPA molecule. These complexes reflect the progressive association of distinct DNA-binding domains present in the RPA heterotrimeric structure. Binding is coupled to significant conformational changes within RPA that are observable at the microscopic level. Presumably, the different conformations of free and ssDNA-bound RPA allow the protein to selectively interact with factors such as ATR-ATRIP when bound to DNA.

Literature References

PubMed ID	Title	Journal	Year
11157767	Structure of the major single-stranded DNA-binding domain of replication protein A suggests a dynamic mechanism for DNA binding Bochkareva, E, Belegu, V, Korolev, S, Bochkarev, A	EMBO J	2001
10473346	Replication protein A (RPA): the eukaryotic SSB. Iftode, C, Daniely, Y, Borowiec, JA	Crit Rev Biochem Mol Biol	1999
8196638	Human replication protein A binds single-stranded DNA in two distinct complexes Blackwell, LJ, Borowiec, JA	Mol Cell Biol	1994
11927569	Structure of the RPA trimerization core and its role in the multistep DNA-binding mechanism of RPA Bochkareva, E, Korolev, S, Lees-Miller, SP, Bochkarev, A	EMBO J	2002