Reactome | Elongation, Polyadenylation and Termination

Elongation, Polyadenylation and Termination

Stable Identifier

R-HSA-168301

Type

Reaction [omitted]

Species

Homo sapiens

Related Species

Influenza A virus

Compartment

nucleoplasm

ReviewStatus

5/5

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Disease (Homo sapiens)

- Infectious disease (Homo sapiens)
  - Viral Infection Pathways (Homo sapiens)
    - Influenza Infection (Homo sapiens)
      - Influenza Viral RNA Transcription and Replication (Homo sapiens)
        
        Viral Messenger RNA Synthesis (Homo sapiens)
        
        Elongation, Polyadenylation and Termination (Homo sapiens)

General

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Elongation, Polyadenylation and Termination

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Catalyzed by the RNA polymerase activity of the viral PB1 subunit, an mRNA complementary to the bound vRNA is synthesized (Plotch, 1977). PA and PB2 move down the growing mRNA in complex with PB1, with PB2 possibly dissociating from the cap (Braam, 1983). However, the 5’ end of the vRNA may remain bound during elongation as the template is threaded through in a 3’ to 5’ direction until a polyadenylation signal is encountered (Poon, 1998; Zheng, 1999).
A poly-uridine sequence motif, consisting in most cases of 5-7 U residues, abuts the "panhandle" duplex structure in the vRNA; this sequence is approximately 16 nucleotides from the 5' end of this RNA duplex structure within the vRNA promoter. Encountering this signal, the viral RNA polymerase stutters, leading to the synthesis of a poly-A tail on the viral mRNA (Robertson, 1981; Luo, 1991; Li,1994; Poon, 1998; Zheng et al. 1999).

Literature References

PubMed ID	Title	Journal	Year
6616622	Molecular model of a eucaryotic transcription complex: functions and movements of influenza P proteins during capped RNA-primed transcription Braam, J, Ulmanen, I, Krug, RM	Cell	1983
10233995	Influenza A virus RNA polymerase has the ability to stutter at the polyadenylation site of a viral RNA template during RNA replication Zheng, H, Lee, HA, Palese, P, Garcia-Sastre, A	J Virol	1999
833924	Influenza virion transcriptase: synthesis in vitro of large, polyadenylic acid-containing complementary RNA Plotch, SJ, Krug, RM	J Virol	1977
9733864	The RNA polymerase of influenza virus, bound to the 5' end of virion RNA, acts in cis to polyadenylate mRNA Poon, LL, Pritlove, DC, Sharps, J, Brownlee, GG	J Virol	1998
2033659	The polyadenylation signal of influenza virus RNA involves a stretch of uridines followed by the RNA duplex of the panhandle structure Luo, GX, Luytjes, W, Enami, M, Palese, P	J Virol	1991
7241649	Polyadenylation sites for influenza virus mRNA Robertson, JS, Schubert, M, Lazzarini, RA	J Virol	1981

Participants

Input

Output

Participates

as an event of

Viral Messenger RNA Synthesis (Homo sapiens)

Event Information

Go Biological Process

viral transcription (0019083)

Catalyst Activity

RNA-directed RNA polymerase activity of Viral Polymerase [nucleoplasm]

Physical Entity

Viral Polymerase [nucleoplasm] (Influenza A virus)

Activity

RNA-directed RNA polymerase activity (GO:0003968)

Disease

Name	Identifier	Synonyms
influenza	DOID:8469	Influenza with other manifestations (disorder), Influenza with other manifestations NOS (disorder), flu, Influenza with other manifestations, Influenza with non-respiratory manifestation (disorder), influenza with non-respiratory manifestation

Cross References

Mondo

0005812

Authored

Bortz, E (2007-02-13)

Garcia-Sastre, A (2007-02-13)

Reviewed

Squires, B (2007-02-13)

Created