Transesterification to connect viral DNA 3' ends to host DNA 5' ends

Stable Identifier
R-HSA-164523
Type
Reaction [transition]
Species
Homo sapiens
Related Species
Human immunodeficiency virus 1
Compartment
nucleoplasm
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Transesterification to connect viral DNA 3' ends to host DNA 5' ends
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The first chemical step of integration involves a single step transesterification, in which the recessed 3' hydroxyl of the viral DNA becomes covalently joined to a protruding 5' end in the target DNA. This step at the same time cleaves the target DNA.

Literature References
PubMed ID Title Journal Year
8883604 HIV cDNA integration: molecular biology and inhibitor development

Farnet, CM, Bushman, FD

AIDS 1996
  Retroviruses

Coffin, JM, Hughes, SH, Varmus, HE

  1997
  Genetic Engineering: Principles and Methods

Setlow, JK

  1998
Participants
Input
Output
Participant Of
hasEvent
Catalyst Activity
Catalyst Activity
Title
integrase activity of IN:viral DNA bound to host genomic DNA with staggered ends [nucleoplasm]
Physical Entity
IN:viral DNA bound to host genomic DNA with staggered ends [nucleoplasm]
Activity
integrase activity (0008907)
Disease
Name Identifier Synonyms
Human immunodeficiency virus infectious disease 526 HIV infection
Authored
Gopinathrao, G  (2006-05-19)
D'Eustachio, P  (2006-05-19)
Reviewed
Bushman, FD  (2006-10-31)
Created
D'Eustachio, P  (2005-05-24)
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