Transesterification to connect viral DNA 3' ends to host DNA 5' ends

Stable Identifier
R-HSA-164523
Type
Reaction [transition]
Species
Homo sapiens
Related Species
Human immunodeficiency virus 1
Compartment
nucleoplasm
ReviewStatus
5/5
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Transesterification to connect viral DNA 3' ends to host DNA 5' ends
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The first chemical step of integration involves a single step transesterification, in which the recessed 3' hydroxyl of the viral DNA becomes covalently joined to a protruding 5' end in the target DNA. This step at the same time cleaves the target DNA.
Literature References
PubMed ID Title Journal Year
  Retroviruses

Varmus, HE, Hughes, SH, Coffin, JM

  1997
8883604 HIV cDNA integration: molecular biology and inhibitor development

Bushman, FD, Farnet, CM

AIDS 1996
  Genetic Engineering: Principles and Methods

Setlow, JK

  1998
Participants
Input
Output
Participates
as an event of
Catalyst Activity

integrase activity of IN:viral DNA bound to host genomic DNA with staggered ends [nucleoplasm]

Physical Entity
IN:viral DNA bound to host genomic DNA with staggered ends [nucleoplasm] (Homo sapiens)
Activity
integrase activity (GO:0008907)
Disease
Name Identifier Synonyms
Human immunodeficiency virus infectious disease DOID:526 HIV infection
Authored
D'Eustachio, P  (2006-05-19)
Gopinathrao, G  (2006-05-19)
Reviewed
Bushman, FD  (2006-10-31)
Created
D'Eustachio, P  (2005-05-24)
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