Reactome | DHNTP is dephosphorylated by PTPS to PTHP

DHNTP is dephosphorylated by PTPS to PTHP

Stable Identifier

R-HSA-1474184

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

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DHNTP is dephosphorylated by PTPS to PTHP

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6-pyruvoyl tetrahydrobiopterin synthase (PTPS) (Takikawa et al. 1986) catalyses the second step in BH4 biosynthesis, the dephosphorylation of DHNTP to 6-pyruvoyl-tetrahydropterin (PTHP). PTPS is believed to function as a homohexamer (Nar et al. 1994, Bürgisser et al. 1994) and has a requirement for Zn2+ (one Zn2+ ion bound per subunit) and Mg2+ ions for activity (Bürgisser et al. 1995). The phosphorylation of Ser-19 is an essential modification for enzyme activity (Scherer-Oppliger et al. 1999).

Literature References

PubMed ID	Title	Journal	Year
7563095	6-Pyruvoyl tetrahydropterin synthase, an enzyme with a novel type of active site involving both zinc binding and an intersubunit catalytic triad motif; site-directed mutagenesis of the proposed active center, characterization of the metal binding site and modelling of substrate binding Bürgisser, DM, Thöny, B, Redweik, U, Hess, D, Heizmann, CW, Huber, R, Nar, H	J Mol Biol	1995
8137809	Three-dimensional structure of 6-pyruvoyl tetrahydropterin synthase, an enzyme involved in tetrahydrobiopterin biosynthesis Nar, H, Huber, R, Heizmann, CW, Thöny, B, Bürgisser, D	EMBO J	1994
8307017	Expression and characterization of recombinant human and rat liver 6-pyruvoyl tetrahydropterin synthase. Modified cysteine residues inhibit the enzyme activity Bürgisser, DM, Thöny, B, Redweik, U, Hunziker, P, Heizmann, CW, Blau, N	Eur J Biochem	1994
10531334	Serine 19 of human 6-pyruvoyltetrahydropterin synthase is phosphorylated by cGMP protein kinase II Scherer-Oppliger, T, Leimbacher, W, Blau, N, Thöny, B	J Biol Chem	1999
3536512	Biosynthesis of tetrahydrobiopterin. Purification and characterization of 6-pyruvoyl-tetrahydropterin synthase from human liver Takikawa, S, Curtius, HC, Redweik, U, Leimbacher, W, Ghisla, S	Eur J Biochem	1986