UniProt:P04843 RPN1

chain
  • signal peptide:1-23
  • chain:24-607
checksum A2351A9CFABAEB6C
comment
  • FUNCTION Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed:31831667). N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.PATHWAY Protein modification; protein glycosylation.SUBUNIT Component of the oligosaccharyltransferase (OST) complex (PubMed:31831667). OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits (PubMed:23606741, PubMed:25135935, PubMed:31831667). STT3A complex assembly occurs through the formation of 3 subcomplexes. Subcomplex 1 contains RPN1 and TMEM258, subcomplex 2 contains the STT3A-specific subunits STT3A, DC2/OSTC, and KCP2 as well as the core subunit OST4, and subcomplex 3 contains RPN2, DAD1, and OST48. The STT3A complex can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes (By similarity). Interacts with TMEM35A/NACHO (By similarity).INTERACTION Identified by mass spectrometry in melanosome fractions from stage I to stage IV.TISSUE SPECIFICITY Expressed in all tissues tested.PTM Ufmylated by UFL1 in response to endoplasmic reticulum stress, promoting reticulophagy of endoplasmic reticulum sheets.SIMILARITY Belongs to the OST1 family.
crossReference
databaseName UniProt
dbId 63115
description
  • recommendedName: fullName evidence="11"Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 alternativeName: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit alternativeName: Ribophorin I shortName: RPN-I alternativeName: Ribophorin-1
displayName UniProt:P04843 RPN1
geneName
  • RPN1
identifier P04843
isSequenceChanged false
keyword
  • 3D-structure
  • Acetylation
  • Endoplasmic reticulum
  • Glycoprotein
  • Isopeptide bond
  • Membrane
  • Reference proteome
  • Signal
  • Transmembrane
  • Transmembrane helix
  • Ubl conjugation
modified [InstanceEdit:12187927] Wright, Adam, 2024-03-12
name
  • RPN1
otherIdentifier
  • 11742852_a_at
  • 11742853_x_at
  • 11748113_a_at
  • 11749429_a_at
  • 16958864
  • 201011_PM_at
  • 201011_at
  • 2694398
  • 2694399
  • 2694400
  • 2694401
  • 2694403
  • 2694404
  • 2694405
  • 2694407
  • 2694409
  • 2694411
  • 2694412
  • 2694415
  • 2694418
  • 2694419
  • 2694420
  • 33424_at
  • 6184
  • 8090490
  • A_23_P132809
  • A_23_P254340
  • A_24_P63262
  • GE58292
  • GO:0003723
  • GO:0003824
  • GO:0004579
  • GO:0005515
  • GO:0005783
  • GO:0005789
  • GO:0005791
  • GO:0005829
  • GO:0006486
  • GO:0006487
  • GO:0008250
  • GO:0016020
  • GO:0016740
  • GO:0018279
  • GO:0031410
  • GO:0036211
  • GO:0042470
  • GO:0043226
  • GO:1901135
  • HMNXSV003047593
  • ILMN_1660533
  • TC03001757.hg
  • Y00281_at
  • g4506674_3p_at
physicalEntity
referenceDatabase [ReferenceDatabase:2] UniProt
referenceGene
referenceTranscript
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • RPN1_HUMAN
  • B2R5Z0
  • D3DNB6
  • Q68DT1
sequenceLength 607
species [Species:48887] Homo sapiens
url https://purl.uniprot.org/uniprot/P04843
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