Reactome | Removal of SPO11 and Resection of 5' Ends of DNA (yeast)

Removal of SPO11 and Resection of 5' Ends of DNA (yeast)

Stable Identifier

R-SCE-981784

Type

Reaction [omitted]

Species

Saccharomyces cerevisiae

Compartment

nucleoplasm

ReviewStatus

5/5

General

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SVG | | PPTX | SBGN

Removal of SPO11 and Resection of 5' Ends of DNA (yeast)

SPO11 forms a dimer and each subunit cleaves a single strand of DNA, thus creating a double-strand break. After cleaving DNA, a SPO11 subunit remains covalently attached to each 5' end via a tyrosine residue. SPO11 is removed from the DNA by cleavage of single strands 3' to the attached SPO11. The products are a resected 5' end (protruding 3' overhang) and a covalent complex of SPO11 with an oligonucleotide.

Literature References

PubMed ID	Title	Journal	Year
16107854	Endonucleolytic processing of covalent protein-linked DNA double-strand breaks Pan, J, Neale, MJ, Keeney, S	Nature	2005
20150422	Processing of meiotic DNA double strand breaks requires cyclin-dependent kinase and multiple nucleases Guerini, I, Citterio, A, Manfrini, N, Longhese, MP, Lucchini, G	J Biol Chem	2010
15852023	The Rad50 hook domain is a critical determinant of Mre11 complex functions Hohl, M, Petrini, JH, Fleming, JC, Wiltzius, JJW	Nat Struct Mol Biol	2005
9858579	The nuclease activity of Mre11 is required for meiosis but not for mating type switching, end joining, or telomere maintenance Moreau, S, Symington, LS, Ferguson, JR	Mol Cell Biol	1999
18245357	Functional interactions between Sae2 and the Mre11 complex Weil, C, Kim, HS, Harrison, JC, Reger, M, Petrini, JH, Vijayakumar, S, Haber, JE	Genetics	2008
18670132	Sae2p phosphorylation is crucial for cooperation with Mre11p for resection of DNA double-strand break ends during meiotic recombination in Saccharomyces cerevisiae Terasawa, M, Ogawa, H, Ogawa, T, Tsukamoto, Y	Genes Genet Syst	2008