Stable Identifier
Reaction [transition]
Saccharomyces cerevisiae
SVG |   | PPTX  | SBGN

The GET3:ADP dimer bound to a tail-anchored protein interacts with the receptor GET1:GET2 (Schuldiner et al. 2008, Stefer et al. 2011, Wang et al. 2011, Rome et al. 2014, Wang et al. 2014, Zalisko et al. 2017). Docking of GET3 causes a change in conformation of the GET3 dimer to an open state (Stefer et al. 2011) that is stabilized by GET1 (Yamagata et al. 2010, Kubota et al. 2012) and releases the tail-anchored client protein to GET1 of the GET1:GET2 insertase (Wang et al. 2011, Wang et al. 2014) .

Literature References
PubMed ID Title Journal Year
28877464 Tail-Anchored Protein Insertion by a Single Get1/2 Heterodimer

Zalisko, BE, Chan, C, Denic, V, Rock, RS, Keenan, RJ

Cell Rep 2017
25368153 Differential gradients of interaction affinities drive efficient targeting and recycling in the GET pathway

Rome, ME, Chio, US, Rao, M, Gristick, H, Shan, SO

Proc. Natl. Acad. Sci. U.S.A. 2014
20015340 Structural insight into the membrane insertion of tail-anchored proteins by Get3

Yamagata, A, Mimura, H, Sato, Y, Yamashita, M, Yoshikawa, A, Fukai, S

Genes Cells 2010
21719644 Structural basis for tail-anchored membrane protein biogenesis by the Get3-receptor complex

Stefer, S, Reitz, S, Wang, F, Wild, K, Pang, YY, Schwarz, D, Bomke, J, Hein, C, Löhr, F, Bernhard, F, Denic, V, Dötsch, V, Sinning, I

Science 2011
18724936 The GET complex mediates insertion of tail-anchored proteins into the ER membrane

Schuldiner, M, Metz, J, Schmid, V, Denic, V, Rakwalska, M, Schmitt, HD, Schwappach, B, Weissman, JS

Cell 2008
25043001 The Get1/2 transmembrane complex is an endoplasmic-reticulum membrane protein insertase

Wang, F, Chan, C, Weir, NR, Denic, V

Nature 2014
21835666 The mechanism of tail-anchored protein insertion into the ER membrane

Wang, F, Whynot, A, Tung, M, Denic, V

Mol. Cell 2011
22684149 Get1 stabilizes an open dimer conformation of get3 ATPase by binding two distinct interfaces

Kubota, K, Yamagata, A, Sato, Y, Goto-Ito, S, Fukai, S

J. Mol. Biol. 2012
Catalyst Activity

ATPase activity of SBH1:GET3:ADP [cytosol]

Orthologous Events
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