Box C/D snoRNP methylates ribonucleotides in pre-rRNA

Stable Identifier
R-SCE-6793739
Type
Reaction [transition]
Species
Saccharomyces cerevisiae
Compartment
nucleoplasm
ReviewStatus
5/5
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
Box C/D snoRNP methylates ribonucleotides in pre-rRNA
The NOP1 component of box C/D small nucleolar ribonucleoprotein complexes (snoRNPs) methylates the 2' position of the ribose moiety of nucleotides in precursor ribosomal RNA (pre-rRNA) (Kiss-Laszlo et al. 1996, Galardi et al. 2002). Box C/D snoRNPs contain NOP1 (homologous to Fibrillarin in humans), NOP56, NOP58 (NOP5), SNU13 (homologous to 15.5K, NHP2L1 in humans), and a box C/D snoRNA, which guides the snoRNP to the site of methylation by base pairing with 10-21 nucleotides of the pre-rRNA (Schimmang et al. 1989, Kiss-Laszlo et al. 1998, Wu et al. 1998, Lafontaine and Tollervey 1999, Lafontaine and Tollervey 2000, Watkins et al. 2000, Galardi et al. 2002).
Literature References
PubMed ID Title Journal Year
10094313 Nop58p is a common component of the box C+D snoRNPs that is required for snoRNA stability

Lafontaine, DL, Tollervey, D

RNA 1999
9632712 Nop5p is a small nucleolar ribonucleoprotein component required for pre-18 S rRNA processing in yeast

Metcalfe, AC, Chen, S, Brockenbrough, JS, Aris, JP, Wu, P

J. Biol. Chem. 1998
9372940 Nucleolar KKE/D repeat proteins Nop56p and Nop58p interact with Nop1p and are required for ribosome biogenesis

Tollervey, D, Hurt, E, Gautier, T, Bergès, T

Mol. Cell. Biol. 1997
11081632 A common core RNP structure shared between the small nucleoar box C/D RNPs and the spliceosomal U4 snRNP

Lührmann, R, Rosbash, M, Branlant, C, Charpentier, B, Wilm, M, Bachi, A, Ségault, V, Nottrott, S, Watkins, NJ, Fabrizio, P

Cell 2000
8674114 Site-specific ribose methylation of preribosomal RNA: a novel function for small nucleolar RNAs

Henry, Y, Kiss, T, Caizergues-Ferrer, M, Bachellerie, JP, Kiss-László, Z

Cell 1996
2686980 A yeast nucleolar protein related to mammalian fibrillarin is associated with small nucleolar RNA and is essential for viability

Schimmang, T, Tollervey, D, Kern, H, Hurt, EC, Frank, R

EMBO J. 1989
10733567 Synthesis and assembly of the box C+D small nucleolar RNPs

Lafontaine, DL, Tollervey, D

Mol. Cell. Biol. 2000
9451004 Sequence and structural elements of methylation guide snoRNAs essential for site-specific ribose methylation of pre-rRNA

Henry, Y, Kiss, T, Kiss-László, Z

EMBO J. 1998
12215523 Purified box C/D snoRNPs are able to reproduce site-specific 2'-O-methylation of target RNA in vitro

Presutti, C, Scaloni, A, Bozzoni, I, Bachi, A, Galardi, S, Fatica, A

Mol. Cell. Biol. 2002
Participants
Input
Output
Event Information
Go Biological Process
rRNA 2'-O-methylation (0000451)
Catalyst Activity

O-methyltransferase activity of Box C/D snoRNP [nucleoplasm]

Physical Entity
Box C/D snoRNP [nucleoplasm] (Saccharomyces cerevisiae)
Activity
O-methyltransferase activity (GO:0008171)
Orthologous Events
Box C/D snoRNP methylates ribonucleotides in pre-rRNA yielding 2'-O-methylribonucleotides (Homo sapiens)
Authored
May, B  (2015-09-01)
Reviewed
Sharma, S  (2016-02-12)
Vincent, NG  (2016-01-30)
Created
May, B  (2015-09-04)
Cite Us!