CIA Targeting Complex transfers 4Fe-4S clusters to apoproteins

Stable Identifier
R-SCE-2564817
Type
Reaction [omitted]
Species
Saccharomyces cerevisiae
Compartment
cytosol
ReviewStatus
5/5
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
CIA Targeting Complex transfers 4Fe-4S clusters to apoproteins
A complex of four proteins termed CIA targeting complex is involved in the delivery of the Cfd1-Nbp35-bound Fe/S cluster to specific target apoproteins. Nar1 (human IOP1) is similar in sequence to iron-only hydrogenases and binds two [4Fe-4S] clusters. These cofactors are essential for biogenesis but their molecular function is unknown. The WD40 domain protein Cia1 (human CIAO1) forms a propeller-like structure which may serve as a docking site for the other members of the CIA targeting complex. The role of Cia2 (human FAM96B) and Mms19 (human MMS19) is still unclear, but they show some specificity for the delivery of Fe/S clusters into certain target apoproteins. They directly interact with numerous Fe/S target proteins including many proteins involved in nuclear DNA integrity and maintenance such as DNA polymerases (Pol3), helicases of the DNA repair pathway (Rad3) or of telomere maintenance, and primases. Many of these target proteins are relevant to various cancer-related diseases or aging.
Literature References
PubMed ID Title Journal Year
22678362 MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity

Netz, DJ, Pierik, AJ, Lill, R, Sharma, T, Vashisht, AA, Stehling, O, Mascarenhas, J, Wohlschlegel, JA, Jonsson, ZO

Science 2012
22678361 MMS19 links cytoplasmic iron-sulfur cluster assembly to DNA metabolism

Flynn, H, Boulton, SJ, León Ortiz, AM, Gari, K, Skehel, JM, Borel, V

Science 2012
16314508 The essential WD40 protein Cia1 is involved in a late step of cytosolic and nuclear iron-sulfur protein assembly

Pierik, AJ, Lill, R, Balk, J, Aguilar Netz, DJ, Tepper, K

Mol. Cell. Biol. 2005
17937914 Structure of the yeast WD40 domain protein Cia1, a component acting late in iron-sulfur protein biogenesis

Srinivasan, V, Netz, DJ, Pierik, AJ, Webert, H, Lill, R, Michel, H, Mascarenhas, J

Structure 2007
Participants
Input
Output
Catalyst Activity

iron-sulfur transferase activity of NAR1:CIA1:YHR122W:MET18 [cytosol]

Physical Entity
NAR1:CIA1:YHR122W:MET18 [cytosol] (Saccharomyces cerevisiae)
Activity
iron-sulfur transferase activity (GO:0036455)
Orthologous Events
CIA Targeting Complex transfers 4Fe-4S cluster to apoproteins (Homo sapiens)
Authored
Lill, R  (2012-11-07)
Reviewed
Uringa, EJ  (2013-02-05)
Created
May, B  (2012-11-08)
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