perilipin + 3 ATP -> phosphorylated perilipin + 3 ADP

Stable Identifier
R-RNO-163601
Type
Reaction [transition]
Species
Rattus norvegicus
Compartment
cytosol, lipid droplet
ReviewStatus
5/5
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
perilipin + 3 ATP -> phosphorylated perilipin + 3 ADP
Rat perilipin, the major protein at the surfaces of cytosolic lipid particles in adipocytes and steroidogenic cells (Blanchette-Mackie et al. 1995), is phosphorylated by protein kinase A catalytic subunit (Greenberg et al. 1991) on serine residues 81, 223, and 277 (Tansey et al. 2003). All three serine residues and the adjoining sequences that mediate phosphorylation (Cohen 1988) are conserved in mouse perilipin, while only the first and third are conserved in human perilipin. By inference, PKA targets these three mouse and two human serines as well. Phosphorylated perilipin is redistributed on the droplet surfaces (Souza et al. 1998). While two isoforms of rat perilipin protein are found on lipid particles in adipocytes, only the larger isoform appears to regulate lipolysis (Tansey et al 2003). The single human and mouse isoforms of perilipin correspond to the large rat isoform. In mouse 3T3-L1 cells, perilipin phosphorylation requires the presence of caveolin-1 at the surface of the lipid particle (Cohen et al. 2004). This positive regulatory effect of caveolin-1 is inferred for rat and human.
Literature References
PubMed ID Title Journal Year
12477720 Functional studies on native and mutated forms of perilipins. A role in protein kinase A-mediated lipolysis of triacylglycerols.

Jones, JM, Huml, AM, Londos, C, Kimmel, AR, Vogt, R, Brasaemle, DL, Fraser, KA, Davis, KE, Tansey, JT

J Biol Chem 2003
2040638 Perilipin, a major hormonally regulated adipocyte-specific phosphoprotein associated with the periphery of lipid storage droplets

Blanchette-Mackie, EJ, Wek, SA, Londos, C, Egan, JJ, Garty, NB, Greenberg, AS

J Biol Chem 1991
7665999 Perilipin is located on the surface layer of intracellular lipid droplets in adipocytes

Dwyer, NK, Takeda, T, Blanchette-Mackie, EJ, Londos, C, Rondinone, CM, Greenberg, AS, Coxey, RA, Theodorakis, JL, Barber, T

J Lipid Res 1995
Participants
Input
Output
Catalyst Activity

protein serine/threonine kinase activity of PKA catalytic subunit [cytosol]

Physical Entity
PKA catalytic subunit [cytosol] (Rattus norvegicus)
Activity
protein serine/threonine kinase activity (GO:0004674)
This event is regulated
Positively by
Regulator
caveolin-1 [lipid droplet] (Rattus norvegicus)
Orthologous Events
perilipin + 2 ATP -> phosphorylated perilipin + 2 ADP (Homo sapiens)
Authored
D'Eustachio, P  (2005-05-02)
Created
D'Eustachio, P  (2005-05-02)
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