CDK1 phosphorylates Mastl

Stable Identifier
R-NUL-2434198
Type
Reaction [transition]
Species
Homo sapiens
Compartment
nucleoplasm
ReviewStatus
5/5
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
CDK1 phosphorylates Mastl
Phosphorylation of MASTL (GWL) by CDK1 (Cdc2) was established using Mastl (Gwl) purified from Xenopus egg extracts and recombinant human CDK1. Phosphopeptide mapping identified several CDK1 phosphorylation sites in Mastl of which threonine residues T193 (Blake-Hodek et al. 2012), T206 (Blake-Hodek et al. 2012) and T748 (Yu et al. 2006) were found to be functionally important. These threonine residues are conserved and they correspond to T194, T207 and T741 of human MASTL. In addition, phosphorylation of Xenopus Mastl on serines S101 and S883 is important for the mitotic function of Mastl, but these sites, although conserved in Xenopus, human and mouse, do not conform to the CDK1 consensus, and the responsible kinase has not been identified. S883 may represent an autophosphorylation site (Blake-Hodek et al. 2012).
Literature References
PubMed ID Title Journal Year
16600872 Greatwall kinase participates in the Cdc2 autoregulatory loop in Xenopus egg extracts

Yu, J, Li, Z, Goldberg, ML, Galas, S, Zhao, Y

Mol. Cell 2006
22354989 Determinants for activation of the atypical AGC kinase Greatwall during M phase entry

Chen, W, Castilho, PV, Williams, BC, Mao, Y, Goldberg, ML, Blake-Hodek, KA, Yamamoto, TM, Zhao, Y

Mol. Cell. Biol. 2012
Participants
Input
Output
Catalyst Activity

cyclin-dependent protein serine/threonine kinase activity of CCNB1:p-T161-CDK1 [nucleoplasm]

Physical Entity
CCNB1:p-T161-CDK1 [nucleoplasm] (Homo sapiens)
Activity
cyclin-dependent protein serine/threonine kinase activity (GO:0004693)
Orthologous Events
CDK1 phosphorylates MASTL (Homo sapiens)
Authored
Orlic-Milacic, M  (2012-09-04)
Reviewed
Mochida, S  (2012-09-26)
Burgess, A  (2012-09-28)
Created
Orlic-Milacic, M  (2012-08-10)
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