CDK8 phosphorylates xNICD1

Stable Identifier
R-NUL-2065178
Type
Reaction [transition]
Species
Homo sapiens
Compartment
General
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CDK8 phosphorylates xNICD1

TAD and PEST domains of NOTCH intracellular domain contain multiple conserved cyclin-dependent kinase phosphorylation sites. In vitro, recombinant human CDK8 in complex with recombinant human cyclin C (CDK8:CCNC) readily phosphorylates recombinant Xenopus NICD1 (xNICD1). This phosphorylation also occurs when these recombinant proteins are expressed in HeLa cells, and was directly shown to involve conserved serine residues in the PEST domain. Phosphorylation by CDK8 targets xNICD1 for ubiquitination and subsequent degradation, thereby coordinating NICD1 transcriptional activity with NICD1 turnover.

Literature References
PubMed ID Title Journal Year
15546612 Mastermind recruits CycC:CDK8 to phosphorylate the Notch ICD and coordinate activation with turnover

Fryer, CJ, White, JB, Jones, KA

Mol Cell 2004
Participants
Catalyst Activity
Catalyst Activity
Title
cyclin-dependent protein serine/threonine kinase activity of xNOTCH1 Chimeric Coactivator Complex:CDK8:CCNC [nucleoplasm]
Physical Entity
Activity
Orthologous Events
Authored
Reviewed
Created