Removal of Spo11 and Resection of 5' Ends of DNA

Stable Identifier
R-MMU-912357
Type
Reaction [omitted]
Species
Mus musculus
Compartment
nucleoplasm
ReviewStatus
5/5
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
Removal of Spo11 and Resection of 5' Ends of DNA
After cleaving DNA Spo11 remains covalently attached to the 5' ends. Spo11 is removed by cleavage of the DNA and release of Spo11:oligonucleotide complexes (Neale et al. 2005, Pan and Keeney 2009). In yeast, the Mre11:Rad50:Xrs2 complex is required for processing of double-strand breaks introduced by Spo11 (Cherry et al. 2007). The homologous Mre11:Rad50:Nbs1 complex from mouse has not been observed to localize to double-strand breaks made by Spo11 on autosomes. The enzymes responsible for removal of Spo11 and resection of the 3' ends remain unknown.
Literature References
PubMed ID Title Journal Year
16107854 Endonucleolytic processing of covalent protein-linked DNA double-strand breaks

Pan, J, Neale, MJ, Keeney, S

Nature 2005
17291760 The Mre11 complex influences DNA repair, synapsis, and crossing over in murine meiosis

Hunt, PA, Hassold, TJ, Adelman, CA, Petrini, JH, Cherry, SM, Theunissen, JW

Curr Biol 2007
19799184 Detection of SPO11-oligonucleotide complexes from mouse testes

Pan, J, Keeney, S

Methods Mol Biol 2009
Participants
Input
Output
Orthologous Events
MRN:CtIP exonucleolytically hydrolyzes DNA 3' to SPO11 and SPO11:double-strand break dissociates to SPO11:oligonucleotide and resected 5' end (Homo sapiens)
MRN:CtIP endonucleolytically cleaves single-strand DNA 3' to SPO11 (Homo sapiens)
Authored
May, B  (2010-07-03)
Reviewed
Bolcun-Filas, E  (2011-02-25)
Lyndaker, A  (2011-02-25)
Holloway, JK  (2011-02-05)
Schimenti, JC  (2011-02-05)
Strong, E  (2011-02-25)
Cohen, PE  (2011-02-05)
Created
May, B  (2010-07-09)
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