Il12a:Il12b translocates from Golgi lumen to late endosome lumen

Stable Identifier
R-MMU-8950510
Type
Reaction [omitted]
Species
Mus musculus
Compartment
Golgi lumen, endosome lumen
ReviewStatus
5/5
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
Il12a:Il12b translocates from Golgi lumen to late endosome lumen
Interleukin-12 (Il12) translocates from the Golgi to the late endosome.

Il12 has been localized intracellularly in late endocytic vesicles marked by the soluble N-ethylmaleimide-sensitive factor accesory protein receptor (SNARE) termed Vesicle-associated membrane protein 7 (Vamp7).
Immunoelectron microscopy on cells expressing p35-SV5 (recombinant murine Il12a with simian virus 5) revealed that the majority of Il12a was associated with Golgi stacks and nearby tubovesicular membranes representing the trans-Golgi Network (Chiaruttini et al. 2016). A fraction of the protein was contained in vesicles and tubules associated with late-endosomes/lysosomes. This indicates that post-golgi trafficking of bioactive Interleukin-12 heterodimer in dendritic cells utilizes late endocytic vesicles preferentially marked by Vamp7.
Literature References
PubMed ID Title Journal Year
26972013 The SNARE VAMP7 Regulates Exocytic Trafficking of Interleukin-12 in Dendritic Cells

Chiaruttini, G, Piperno, GM, Jouve, M, De Nardi, F, Larghi, P, Peden, AA, Baj, G, Müller, S, Valitutti, S, Galli, T, Benvenuti, F

Cell Rep 2016
Participants
Input
Output
Orthologous Events
IL12A:IL12B translocates from Golgi lumen to late endosome lumen (Homo sapiens)
Authored
Duenas, C  (2016-12-02)
Reviewed
van de Vosse, E  (2017-05-12)
Created
Duenas, C  (2016-12-02)
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