Reactome | Il12a:Il12b translocates from ER lumen to Golgi

Il12a:Il12b translocates from ER lumen to Golgi

Stable Identifier

R-MMU-8950489

Type

Reaction [omitted]

Species

Mus musculus

Compartment

endoplasmic reticulum lumen, Golgi lumen

ReviewStatus

5/5

General

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Il12a:Il12b translocates from ER lumen to Golgi

Interleukin-12 heterodimeric complex formed by Interleukin-12 subunit alpha (Il12a) and Interleukin-12 subunit beta (Il12b), has been localized intracellularly in late endocytic vesicles marked by the Soluble N-ethylmaleimide-sensitive factor accesory protein receptor (SNARE) termed Vesicle-associated membrane protein 7 (Vamp7). All SNAREs colocalized with Interleukin-12 heterodimer in the Golgi area, reflecting the accumulation of newly synthesized protein. Immunoelectron microscopy on cells expressing p35-SV5 (recombinant murine Il12a with simian virus 5) revealed that the majority of Il12a was associated with Golgi stacks and nearby tubovesicular membranes representing the Trans Golgi Network. A fraction of the protein was contained in vesicles and tubules associated with late-endosomes/lysosomes (Chiaruttini et al. 2016).

This reaction is a black box event because we know this Interleukin-12 heterodimeric protein is translocated from Endoplasmic reticulum to Golgi lumen but there is not reported evidence from which compartment and which mechanism is involved to the translocation.

Literature References

PubMed ID	Title	Journal	Year
26972013	The SNARE VAMP7 Regulates Exocytic Trafficking of Interleukin-12 in Dendritic Cells Chiaruttini, G, Piperno, GM, Jouve, M, De Nardi, F, Larghi, P, Peden, AA, Baj, G, Müller, S, Valitutti, S, Galli, T, Benvenuti, F	Cell Rep	2016