Adrbk1 binds pS592, S594, T597, S599 Smo dimer:Csnk1a1

Stable Identifier
R-MMU-5632663
Type
Reaction [binding]
Species
Mus musculus
Compartment
ciliary membrane
ReviewStatus
5/5
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
Adrbk1 binds pS592, S594, T597, S599 Smo dimer:Csnk1a1
Ardbk1 binds to the Smo C-terminal tail as assessed by co-immunoprecipitation from NIH 3T3 cells. Binding is enhanced after the Smo C-terminal tail is phosphorylated by Csnk1a1. Ardbk1 phosphorylates Smo on numerous serine and threonine residues in an in vitro assay, but phosphorylation in the S1 region appears to be the most critical. Phosphorylation by Csnk1a1 and Ardbk1 promotes an open tail conformation within the Smo dimer, activating the protein and promoting downstream signal propagation (Chen et al, 2011; Chen et al, 2010; Zhao et al, 2007).
Literature References
PubMed ID Title Journal Year
21695114 Sonic Hedgehog dependent phosphorylation by CK1? and GRK2 is required for ciliary accumulation and activation of smoothened

Yue, T, Jia, J, Ma, G, Sasai, N, Briscoe, J, Jiang, J, Chen, Y

PLoS Biol. 2011
20844016 G protein-coupled receptor kinase 2 promotes high-level Hedgehog signaling by regulating the active state of Smo through kinase-dependent and kinase-independent mechanisms in Drosophila

Zhao, Y, Jia, J, Li, S, Tong, C, Liu, Y, Wang, B, Jiang, J, Chen, Y

Genes Dev. 2010
17960137 Hedgehog regulates smoothened activity by inducing a conformational switch

Tong, C, Jiang, J, Zhao, Y

Nature 2007
Participants
Input
Output
Orthologous Events
ADRBK1 binds phosphorylated SMO dimer (Homo sapiens)
Authored
Rothfels, K  (2014-10-24)
Reviewed
Liu, Y C  (2014-11-09)
Created
Rothfels, K  (2014-10-28)
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