MeR-Piwil4:2'-O-methyl-piRNA:Tdrd9:Mael:Tdrkh dissociates and MeR-Piwil4:2'-O-methyl-piRNA, Tdrd9, Mael translocate from cytosol to nucleus in unknown association and unknown order of events

Stable Identifier
R-MMU-5606256
Type
Reaction [omitted]
Species
Mus musculus
Compartment
Synonyms
Transport of Piwil4:piRNA to the nucleus
General
SVG |   | PPTX  | SBGN
MeR-Piwil4:2'-O-methyl-piRNA:Tdrd9:Mael:Tdrkh dissociates and MeR-Piwil4:2'-O-methyl-piRNA, Tdrd9, Mael translocate from cytosol to nucleus in unknown association and unknown order of events

Piwil4 is loaded with piRNA in the cytosol and then translocates to the nucleus where it directs transcriptional silencing of cognate loci by an unknown mechanism that results in DNA methylation (Carmell et al. 2007, Kuramochi-Miyagawa et al. 2008). Most cellular Piwil4 is translocated to the nucleus at E16.5 of mouse development (Aravin et al. 2009) and proper localization depends on Piwil2 and Tdrd1 (Reuter et al. 2009). Tdrd9 and Mael interact with Piwil4, are observed in the nucleus, and may play a role in the translocation of Piwil4 (Aravin et al. 2009, Shoji et al. 2009). Loss of Tdrd9, however, does not affect nuclear localization of Piwil4 (Shoji et al. 2009). Loss of Mael delays but does not prevent localization of Piwil4 to the nucleus (Aravin et al. 2009). Tdrkh is required for translocation of Piwil4, however Tdrkh is only observed in the cytosol (Saxe et al. 2013).

Literature References
PubMed ID Title Journal Year
19465913 Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile

Reuter, M, Chuma, S, Tanaka, T, Franz, T, Stark, A, Pillai, RS

Nat. Struct. Mol. Biol. 2009
18381894 DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes

Kuramochi-Miyagawa, S, Watanabe, T, Gotoh, K, Totoki, Y, Toyoda, A, Ikawa, M, Asada, N, Kojima, K, Yamaguchi, Y, Ijiri, TW, Hata, K, Li, E, Matsuda, Y, Kimura, T, Okabe, M, Sakaki, Y, Sasaki, H, Nakano, T

Genes Dev. 2008
17395546 MIWI2 is essential for spermatogenesis and repression of transposons in the mouse male germline

Carmell, MA, Girard, A, van de Kant, HJ, Bourc'his, D, Bestor, TH, de Rooij, DG, Hannon, GJ

Dev. Cell 2007
20011505 Cytoplasmic compartmentalization of the fetal piRNA pathway in mice

Aravin, AA, van der Heijden, GW, CastaƱeda, J, Vagin, VV, Hannon, GJ, Bortvin, A

PLoS Genet. 2009
20059948 The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline

Shoji, M, Tanaka, T, Hosokawa, M, Reuter, M, Stark, A, Kato, Y, Kondoh, G, Okawa, K, Chujo, T, Suzuki, T, Hata, K, Martin, SL, Noce, T, Kuramochi-Miyagawa, S, Nakano, T, Sasaki, H, Pillai, RS, Nakatsuji, N, Chuma, S

Dev. Cell 2009
23714778 Tdrkh is essential for spermatogenesis and participates in primary piRNA biogenesis in the germline

Saxe, JP, Chen, M, Zhao, H, Lin, H

EMBO J. 2013
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