Complexed Piwil2:2'-O-methyl-piRNA cleaves transposon RNA

Stable Identifier
Reaction [binding]
Mus musculus
SVG |   | PPTX  | SBGN
Complexed Piwil2:2'-O-methyl-piRNA cleaves transposon RNA

Piwil2 (Mili) bound to a piRNA cleaves target RNAs complementary to the piRNA (De Fazio et al. 2011, Di Giacomo et al. 2013, Reuter and Pillai 2014). The cleaved RNA can either be transferred to another Piwil2 as part of the "ping pong cycle" that generates secondary piRNAs or the cleaved RNA can be transferred to Piwil4 (Miwi2), which then transits to the nucleus to transcriptionally silence loci complementary to the piRNA.

Literature References
PubMed ID Title Journal Year
23706823 Multiple epigenetic mechanisms and the piRNA pathway enforce LINE1 silencing during adult spermatogenesis

Di Giacomo, M, Comazzetto, S, Saini, H, De Fazio, S, Carrieri, C, Morgan, M, Vasiliauskaite, L, Benes, V, Enright, AJ, O'Carroll, D

Mol. Cell 2013
22020280 The endonuclease activity of Mili fuels piRNA amplification that silences LINE1 elements

De Fazio, S, Bartonicek, N, Di Giacomo, M, Abreu-Goodger, C, Sankar, A, Funaya, C, Antony, C, Moreira, PN, Enright, AJ, O'Carroll, D

Nature 2011
24178560 Analysis of small RNA-guided endonuclease activity in endogenous Piwi protein complexes from mouse testes

Reuter, M, Pillai, RS

Methods Mol. Biol. 2014
Orthologous Events
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