Reactome | Unknown nuclease cleaves pre-piRNA in 4xMeR-Piwil1:pre-piRNA:Tdrd6:Tdrkh

Unknown nuclease cleaves pre-piRNA in 4xMeR-Piwil1:pre-piRNA:Tdrd6:Tdrkh

Stable Identifier

R-MMU-5605344

Type

Reaction [omitted]

Species

Mus musculus

Compartment

cytosol

ReviewStatus

5/5

General

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Unknown nuclease cleaves pre-piRNA in 4xMeR-Piwil1:pre-piRNA:Tdrd6:Tdrkh

After binding Piwil1 (Miwi in mouse, HIWI in human) the 3' end of the pre-piRNA is trimmed by an unknown nuclease (Aravin et al. 2006, Grivna et al. 2006, Aravin et al. 2008, Vourekas et al. 2012). The final size of the piRNA appears to be determined by the particular PIWI protein with which it is associated (reviewed in Siomi et al. 2011). The existence of a magnesium-dependent trimming activity is inferred from in vitro reactions using proteins of silkworm (Bombyx mori) ovary cells (Kawaoka et al. 2011). Piwil1 and Tdrd6 are located in the chromatoid body. Both Tdrd6 and Tdrkh are associated with PIWIL1 in adult testes but only Tdrkh is present in embryonic prospermatogonia (Chen et al. 2009). Tdrkh is required for spermatogenesis and appears to participate in trimming of the 3' end of pre-piRNAs (Saxe et al. 2013).

Literature References

PubMed ID	Title	Journal	Year
21427766	PIWI-interacting small RNAs: the vanguard of genome defence Siomi, MC, Pezic, D, Aravin, AA, Sato, K	Nat. Rev. Mol. Cell Biol.	2011
21237665	Recognition of 2'-O-methylated 3'-end of piRNA by the PAZ domain of a Piwi protein Kirkpatrick, JP, Andrade-Navarro, MA, Rocha, EA, Pillai, RS, Simon, B, Eckhardt, S, Carlomagno, T, Sehr, P, Reuter, M	Structure	2011
16751776	A germline-specific class of small RNAs binds mammalian Piwi proteins Carmell, MA, Hannon, GJ, Sachidanandam, R, Girard, A	Nature	2006
19918066	Mouse Piwi interactome identifies binding mechanism of Tdrkh Tudor domain to arginine methylated Miwi Pawson, T, Min, J, Guo, Y, Jin, J, James, DA, Park, JG, Chen, C, Gish, G, Xu, C, Tenaglia, E, Adams-Cioaba, MA	Proc. Natl. Acad. Sci. U.S.A.	2009
22842725	Mili and Miwi target RNA repertoire reveals piRNA biogenesis and function of Miwi in spermiogenesis Gregory, BD, Maragkakis, M, Mourelatos, Z, Alexiou, P, Zheng, Q, Vourekas, A, Kirino, Y	Nat. Struct. Mol. Biol.	2012
21925389	3' end formation of PIWI-interacting RNAs in vitro Katsuma, S, Kawaoka, S, Tomari, Y, Izumi, N	Mol. Cell	2011
22121019	Miwi catalysis is required for piRNA amplification-independent LINE1 transposon silencing Hosokawa, M, Pillai, RS, Berninger, P, Chuma, S, Funaya, C, Reuter, M, Sachidanandam, R, Antony, C, Shah, H	Nature	2011
23714778	Tdrkh is essential for spermatogenesis and participates in primary piRNA biogenesis in the germline Chen, M, Lin, H, Saxe, JP, Zhao, H	EMBO J.	2013