Tet1,2,3 oxidizes 5-formylcytosine to 5-carboxylcytosine

Stable Identifier
Reaction [transition]
Mus musculus
SVG |   | PPTX  | SBGN
Tet1,2,3 oxidizes 5-formylcytosine to 5-carboxylcytosine

Tet1, Tet2, and Tet3 each oxidize 5-formylcytosine (5-fC) in DNA using molecular oxygen and 2-oxoglutarate to yield 5-carboxylcytosine (5-caC), carbon dioxide, and succinate (He et al. 2011, Ito et al. 2011, Crawford et al. 2016). 5-caC is observed at major satellite repeats (Shen et al. 2013). 5-caC is generated in zygotes and its persistence indicates it may be passively removed by dilution during DNA replication (Inoue et al. 2011).

Literature References
PubMed ID Title Journal Year
23602152 Genome-wide analysis reveals TET- and TDG-dependent 5-methylcytosine oxidation dynamics

Diep, D, Fung, HL, Wu, H, Shen, L, Zhang, K, Yamaguchi, S, D'Alessio, AC, Zhang, Y

Cell 2013
22124233 Generation and replication-dependent dilution of 5fC and 5caC during mouse preimplantation development

Shen, L, Dai, Q, Zhang, Y, He, C, Inoue, A

Cell Res. 2011
21817016 Tet-mediated formation of 5-carboxylcytosine and its excision by TDG in mammalian DNA

Li, BZ, Tang, Q, Sun, Y, Jia, Y, Li, X, Chen, Z, Song, CX, Wang, Y, Li, Z, Liu, P, Zhang, K, Ding, J, Li, L, Dai, Q, Xu, GL, He, C, He, YF

Science 2011
21778364 Tet proteins can convert 5-methylcytosine to 5-formylcytosine and 5-carboxylcytosine

Shen, L, Ito, S, Dai, Q, Zhang, Y, He, C, Wu, SC, Swenberg, JA, Collins, LB

Science 2011
26734843 Tet2 Catalyzes Stepwise 5-Methylcytosine Oxidation by an Iterative and de novo Mechanism

Garcia, BA, Crawford, DJ, Kohli, RM, Liu, MY, Cao, XJ, Nabel, CS

J. Am. Chem. Soc. 2016
Event Information
Catalyst Activity

methylcytosine dioxygenase activity of Tet1,2,3 [nucleoplasm]

Orthologous Events
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