Translocation of Glut4 Vesicle and Docking at the Plasma Membrane

Stable Identifier
Reaction [omitted]
Mus musculus
SVG |   | PPTX  | SBGN
Translocation of Glut4 Vesicle and Docking at the Plasma Membrane

Glut4 (Slc2a4) initially translocates from endosomes to insulin-responsive vesicles (IRVs, GSVs). Rab11 appears to play a role in this process. IRVs bearing Glut4 are then translocated across the cortical actin network to the plasma membrane. Unconventional myosin 5A (Myo5a) interacts with RAB10 or RAB8A on the vesicle and participates in transport of the IRV. Myosin 1C appears to act close to the plasma membrane and may facilitate fusion of the vesicle with the plasma membrane. RAB:GTP complexes coupled to the vesicles may interact with myosins to regulate their activity. Non-muscle myosin IIA (Myh9) appears to interact with the SNAP23 complex to dock the IRV at the inner membrane face.

Literature References
PubMed ID Title Journal Year
19968963 Myosin IIA participates in docking of Glut4 storage vesicles with the plasma membrane in 3T3-L1 adipocytes

Chung, le TK, Hosaka, T, Harada, N, Jambaldorj, B, Fukunaga, K, Nishiwaki, Y, Teshigawara, K, Sakai, T, Nakaya, Y, Funaki, M

Biochem. Biophys. Res. Commun. 2010
15883051 A novel role for myosin II in insulin-stimulated glucose uptake in 3T3-L1 adipocytes

Steimle, PA, Fulcher, FK, Patel, YM

Biochem. Biophys. Res. Commun. 2005
22908308 Rab10 and myosin-Va mediate insulin-stimulated GLUT4 storage vesicle translocation in adipocytes

Chen, Y, Wang, Y, Zhang, J, Deng, Y, Jiang, L, Song, E, Wu, XS, Hammer, JA, Xu, T, Lippincott-Schwartz, J

J. Cell Biol. 2012
21127070 Myo1c regulates glucose uptake in mouse skeletal muscle

Toyoda, T, An, D, Witczak, CA, Koh, HJ, Hirshman, MF, Fujii, N, Goodyear, LJ

J Biol Chem 2011
17765682 Activation of RalA is required for insulin-stimulated Glut4 trafficking to the plasma membrane via the exocyst and the motor protein Myo1c

Chen, XW, Leto, D, Chiang, SH, Wang, Q, Saltiel, AR

Dev Cell 2007
12687004 The exocyst complex is required for targeting of Glut4 to the plasma membrane by insulin

Inoue, M, Chang, L, Hwang, J, Chiang, SH, Saltiel, AR

Nature 2003
15550383 Evidence for a role of the exocyst in insulin-stimulated Glut4 trafficking in 3T3-L1 adipocytes

Ewart, MA, Clarke, M, Kane, S, Chamberlain, LH, Gould, GW

J. Biol. Chem. 2005
29089333 Electrical pulse stimulation induces GLUT4 glucose transporter translocation in C2C12 myotubes that depends on Rab8A, Rab13 and Rab14

Li, Z, Yue, Y, Hu, F, Zhang, C, Ma, X, Li, N, Qiu, L, Fu, M, Chen, L, Yao, Z, Bilan, PJ, Klip, A, Niu, W

Am. J. Physiol. Endocrinol. Metab. 2017
16525015 Compartmentalization of the exocyst complex in lipid rafts controls Glut4 vesicle tethering

Inoue, M, Chiang, SH, Chang, L, Chen, XW, Saltiel, AR

Mol. Biol. Cell 2006
24374234 Regulation of myosin IIA and filamentous actin during insulin-stimulated glucose uptake in 3T3-L1 adipocytes

Stall, R, Ramos, J, Kent Fulcher, F, Patel, YM

Exp. Cell Res. 2014
18773891 Dual role for myosin II in GLUT4-mediated glucose uptake in 3T3-L1 adipocytes

Fulcher, FK, Smith, BT, Russ, M, Patel, YM

Exp. Cell Res. 2008
Catalyst Activity
Catalyst Activity
microfilament motor activity of p-S1650-Myo5a:F-actin [cytosol]
Physical Entity
microfilament motor activity of GGC-Rala:GTP:Myo1c:Calm1:F-actin [cytoplasmic vesicle membrane]
Physical Entity
This event is regulated
Orthologous Events