RNASEH1 cleaves RNA primer from nascent L strand, leaving RNA dinucleotides

Stable Identifier
R-HSA-9915449
Type
Reaction [transition]
Species
Homo sapiens
Compartment
mitochondrial matrix
ReviewStatus
5/5
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RNASEH1 cleaves RNA primer from nascent L strand, leaving RNA dinucleotides
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RNASEH1 cleaves the RNA primer from the 5' end of the nascent L strand (Al-Behadili et al. 2018, also inferred from knockout of Rnaseh1 in mice in Misic et al. 2022), leaving 1-3 ribonucleotides remaining at the 5' end (Lima et al. 2007, Al-Behadili et al. 2018). Absence of RNASEH1 in mouse fibroblasts causes retention of RNA primers in the OriH region of the replicated H strand and in the OriL region of the replicated L strand (Holmes et al. 2015)
Literature References
PubMed ID Title Journal Year
17028158 Human RNase H1 discriminates between subtle variations in the structure of the heteroduplex substrate

Lima, WF, Rose, JB, Nichols, JG, Wu, H, Migawa, MT, Wyrzykiewicz, TK, Siwkowski, AM, Crooke, ST

Mol Pharmacol 2007
30102370 A two-nuclease pathway involving RNase H1 is required for primer removal at human mitochondrial OriL

Al-Behadili, A, Uhler, JP, Berglund, AK, Peter, B, Doimo, M, Reyes, A, Wanrooij, S, Zeviani, M, Falkenberg, M

Nucleic Acids Res 2018
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Catalyst Activity

RNA-DNA hybrid ribonuclease activity of RNASEH1:Mg2+ [mitochondrial matrix]

Physical Entity
RNASEH1:Mg2+ [mitochondrial matrix] (Homo sapiens)
Activity
RNA-DNA hybrid ribonuclease activity (GO:0004523)
Authored
May, B  (2024-07-07)
Reviewed
Wu, CC  (2024-10-27)
Created
May, B  (2024-07-07)
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