POLG in the replisome polymerizes the L strand

Stable Identifier
R-HSA-9914498
Type
Reaction [omitted]
Species
Homo sapiens
Compartment
mitochondrial matrix
ReviewStatus
5/5
Locations in the PathwayBrowser
Expand all
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
POLG in the replisome polymerizes the L strand
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout
POLG:POLG2 (POLgamma) initiates polymerization of L strand DNA at the 3' hydroxyl group of an RNA primer produced by POLRMT (Wanrooij et al. 2008). Cleavage of the RNA primer by RNASEH1 is evidently not required (Wanrooij et al. 2008), unlike the case of the RNA primer for synthesis of the H strand. The template strand, the parental H strand, is already single-stranded and bound to tetramers of SSBP1, therefore the DNA helicase TWNK (PEO1) is not believed to be required for synthesis of the L strand.
Literature References
PubMed ID Title Journal Year
18685103 Human mitochondrial RNA polymerase primes lagging-strand DNA synthesis in vitro

Wanrooij, S, Fusté, JM, Farge, G, Shi, Y, Gustafsson, CM, Falkenberg, M

Proc Natl Acad Sci U S A 2008
34423452 Mechanism of transcription initiation and primer generation at the mitochondrial replication origin OriL

Sarfallah, A, Zamudio-Ochoa, A, Anikin, M, Temiakov, D

EMBO J 2021
16574152 A novel processive mechanism for DNA synthesis revealed by structure, modeling and mutagenesis of the accessory subunit of human mitochondrial DNA polymerase

Fan, L, Kim, S, Farr, CL, Schaefer, KT, Randolph, KM, Tainer, JA, Kaguni, LS

J Mol Biol 2006
Participants
Input
Output
Participates
as an event of
Event Information
Go Biological Process
mitochondrial DNA replication (0006264)
Catalyst Activity

DNA-directed DNA polymerase activity of L strand primer:Mitochondrial L strand replisome:Mitochondrial H strand replisome:H strand RNA primer:nascent H strand DNA [mitochondrial matrix]

Physical Entity
L strand primer:Mitochondrial L strand replisome:Mitochondrial H strand replisome:H strand RNA primer:nascent H strand DNA [mitochondrial matrix] (Homo sapiens)
Activity
DNA-directed DNA polymerase activity (GO:0003887)
Orthologous Events
POLG in the replisome polymerizes the L strand (Bos taurus)
POLG in the replisome polymerizes the L strand (Caenorhabditis elegans)
POLG in the replisome polymerizes the L strand (Canis familiaris)
POLG in the replisome polymerizes the L strand (Drosophila melanogaster)
POLG in the replisome polymerizes the L strand (Gallus gallus)
POLG in the replisome polymerizes the L strand (Mus musculus)
POLG in the replisome polymerizes the L strand (Rattus norvegicus)
POLG in the replisome polymerizes the L strand (Sus scrofa)
POLG in the replisome polymerizes the L strand (Xenopus tropicalis)
Authored
May, B  (2024-06-17)
Reviewed
Wu, CC  (2024-10-27)
Created
May, B  (2024-06-24)
Cite Us!