Reactome | POLRMT and POLG bind the mitochondrial L strand replication origin (OriL)

POLRMT and POLG bind the mitochondrial L strand replication origin (OriL)

Stable Identifier

R-HSA-9914404

Type

Reaction [binding]

Species

Homo sapiens

Compartment

mitochondrial matrix

ReviewStatus

5/5

Locations in the PathwayBrowser

Expand all

General

SBML | | PDF

SVG | | PPTX | SBGN

POLRMT and POLG bind the mitochondrial L strand replication origin (OriL)

Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

After replication of the H strand proceeds past OriL of the mitochondrial chromosome, OriL becomes single stranded and a stem-loop structure in OriL adopts a configuration that initiates transcription by POLRMT to create the RNA primer that initiates replication of the L strand by POLG:POLG2 (Fusté et al. 2010, Sarfallah et al. 2021, inferred from mice in vivo in Wanrooij et al. 2012). Prior to initiation, POLRMT, POLG:POLG2, and OriL form a ternary complex (Sarfallah et al. 2021).

Literature References

PubMed ID	Title	Journal	Year
20129056	Mitochondrial RNA polymerase is needed for activation of the origin of light-strand DNA replication Fusté, JM, Wanrooij, S, Jemt, E, Granycome, CE, Cluett, TJ, Shi, Y, Atanassova, N, Holt, IJ, Gustafsson, CM, Falkenberg, M	Mol Cell	2010
34423452	Mechanism of transcription initiation and primer generation at the mitochondrial replication origin OriL Sarfallah, A, Zamudio-Ochoa, A, Anikin, M, Temiakov, D	EMBO J	2021