Reactome | Maturation of the canonical 20S core particle

Maturation of the canonical 20S core particle

Stable Identifier

R-HSA-9908101

Type

Reaction [omitted]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

3/5

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Maturation of the canonical 20S core particle

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Upon dimerization of two half-proteasomes to form the preholoproteasome, the active site–bearing beta-subunits beta-1 (PSMB6), beta-2 (PSMB7), and beta-5 (PSMB5), undergo autocatalytic cleavage of their propeptides, generating the mature catalytic sites and completing the 20S core particle (20S CP) assembly (Schmidtke et al. 1997; Hirano et al. 2008; Adolf et al. 2024; reviewed in Tomko and Hochstrasser 2013). Although beta-6 (PSMB1) and beta-7 (PSMB4) also undergo proteolytic cleavage, their cleavage is not of crucial importance for the catalytic function of the 20S core particle (Hirano et al. 2008). PSMG1, PSMG2 and POMP undergo proteolytic degradation mediated by the proteolytic subunits of the inner ring, as reported in yeast (reviewed in Tomko and Hochstrasser 2013) and confirmed in human cells (Burri et al. 2000: absence of POMP from mature 20S CPs; Hirano et al. 2005: degradation of PSMG1 and PSMG2; Hirano et al. 2006: degradation of POMP, PSMG1 and PSMG2; Adolf et al. 2024: degradation of POMP precedes the degradation of PSMG1 and PSMG2).

Literature References

PubMed ID	Title	Journal	Year
16251969	A heterodimeric complex that promotes the assembly of mammalian 20S proteasomes Nagane, R, Murata, S, Natsume, T, Hirano, Y, Hendil, KB, Tanaka, K, Yashiroda, H, Iemura, S, Hioki, Y	Nature	2005
10973495	Identification and characterization of a mammalian protein interacting with 20S proteasome precursors Lévy, F, Boehm, U, Klamp, T, Burri, L, Dohmen, RJ, Höckendorff, J	Proc Natl Acad Sci U S A	2000
17189198	Cooperation of multiple chaperones required for the assembly of mammalian 20S proteasomes Kasahara, M, Murata, S, Natsume, T, Hirano, Y, Kishimoto, T, Hendil, KB, Tanaka, K, Iemura, S, Niwa, S, Hayashi, H	Mol Cell	2006
9149144	Maturation of mammalian 20 S proteasome: purification and characterization of 13 S and 16 S proteasome precursor complexes Kloetzel, PM, Schmidtke, G, Schmidt, M	J Mol Biol	1997
38600324	Visualizing chaperone-mediated multistep assembly of the human 20S proteasome Hanna, J, Goodall, EA, Schulman, BA, Adolf, F, von Gronau, S, Harper, JW, Walsh, RM, Rawson, S, Du, J	Nat Struct Mol Biol	2024