Reactome | PSMD9 binds PSMC3:PSMC6

PSMD9 binds PSMC3:PSMC6

Stable Identifier

R-HSA-9907965

Type

Reaction [binding]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

3/5

Locations in the PathwayBrowser

Expand all

General

SBML | | PDF

SVG | | PPTX | SBGN

Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

The subcomplex of the proteasome 19S regulatory particle (19S RP) base, the PSMC3:PSMC6 dimer (orthologous to the yeast Rpt5:Rpt4 dimer) binds the chaperone protein PSMD9 (orthologous to yeast Nas2) (reviewed in Tomko and Hochstrasser 2013). Formation of the endogenous heterotrimer between PSMD9 (Nas2, also known as p27), PSMC3 (Rpt5), and PSMC6 (Rpt4) was demonstrated in human embryonic kidney cell line HEK293T (Kaneko et al. 2009). Using recombinant human proteins, it was shown that PSMD9 directly interacts with PSMC3 but not PSCM6 (Kaneko et al. 2009). Knockdown of PSMD9 decreases protein levels of both PSMC3 and PSMC6 (Kaneko et al. 2009).

Literature References

PubMed ID	Title	Journal	Year
19490896	Assembly pathway of the Mammalian proteasome base subcomplex is mediated by multiple specific chaperones Murata, S, Natsume, T, Tanaka, K, Hamazaki, J, Kaneko, T, Furuyama, K, Iemura, S, Sasaki, K	Cell	2009