Reactome | mTORC2 phosphorylates AKT1 on serine-473

mTORC2 phosphorylates AKT1 on serine-473

Stable Identifier

R-HSA-9860800

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol, plasma membrane

ReviewStatus

5/5

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mTORC2 phosphorylates AKT1 on serine-473

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Suppression of the RICTOR subunit of the mTORC2 complex blocks flow-induced phosphorylation of serine-473 of AKT1 (Jin et al. 2021). The mTORC2 complex has been shown to directly phosphorylate AKT1 (Sarbassov et al. 2005). mTORC2 is likely activated by PI3K signaling as PI3K activity is required for phosphorylation of serine-473 of AKT1 in response to shear flow stress (Jin et al. 2021). AKT1 phosphorylated on serine-308 and serine-473 is located at the plasma membrane at cell-cell junctions in endothelial cells experiencing unidirectional laminar shear stress, but it is perinuclear in cells experiencing disturbed flow (Melchior and Frangos 2014).

Literature References

PubMed ID	Title	Journal	Year
15718470	Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex Sarbassov, DD, Guertin, DA, Ali, SM, Sabatini, DM	Science	2005
23913776	Distinctive subcellular Akt-1 responses to shear stress in endothelial cells Melchior, B, Frangos, JA	J Cell Biochem	2014
34499618	Protein kinase N2 mediates flow-induced endothelial NOS activation and vascular tone regulation Jin, YJ, Chennupati, R, Li, R, Liang, G, Wang, S, Iring, A, Graumann, J, Wettschureck, N, Offermanns, S	J Clin Invest	2021