MITF-M dimer binds RAB27A gene

Stable Identifier
R-HSA-9856466
Type
Reaction [binding]
Species
Homo sapiens
Compartment
nucleoplasm
ReviewStatus
5/5
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MITF-M dimer binds RAB27A gene
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MITF-M binds to the promoter of the RAB27A gene as determined by EMSA and ChIP in human and mouse cells (Chiaverini et al, 2008, Strub et al, 2011; reviewed in Cheli et al, 2010; Goding and Arnheiter, 2019). MITF-M binding to two E box elements at position-58 to -53 and -47 to -42 drives alpha-MSH and forskolin-responsive expression of RAB27A in mouse and human cells (Chiaverini et al, 2008).
Active GTP:bound RAB27A forms a tripartite complex with MYO5A (another MITF-M target gene) and MLPH (also known as melanophilin) to regulate the localization of melanosomes (Bahadoran et al, 2001; Fukuda et al, 2002; Nagashima et al, 2003; Kuroda and Fukuda, 2004; Alves et al, 2013; Evans et al, 2014; Alves et al, 2017; reviewed in Seabra and Coudrier, 2004; Cheli et al, 2010; Hume and Seabra, 2011). MYO5A interacts with the actin cytoskeleton to localize melanosomes to the ends of dendrites in a manner that also depends on RAB27A and MLPH. Experiments in which MITF-M levels are deliberately manipulated in vitro indicate that MITF-M, by regulating RAB27A, plays a role in melanosome localization (Kuroda and Fukuda, 2004; Chiaverini et al, 2008).
Literature References
PubMed ID Title Journal Year
11266474 Rab27a: A key to melanosome transport in human melanocytes

Casaroli-Marano, R, Bille, K, Ballotti, R, Mantoux, F, Bahadoran, P, Ortonne, JP, Buscà, R, Aberdam, E, Yalman, N, de Saint-Basile, G

J Cell Biol 2001
12062444 Melanophilin directly links Rab27a and myosin Va through its distinct coiled-coil regions

Igarashi, M, Yi, Z, Takeuchi, T, Nagashima, K, Torii, S, Izumi, T, Okamoto, K

FEBS Lett 2002
21936787 Melanosomes on the move: a model to understand organelle dynamics

Seabra, MC, Hume, AN

Biochem Soc Trans 2011
11856727 Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport

Fukuda, M, Kuroda, TS, Mikoshiba, K

J Biol Chem 2002
19995375 Fifteen-year quest for microphthalmia-associated transcription factor target genes

Bertolotto, C, Cheli, Y, Ohanna, M, Ballotti, R

Pigment Cell Melanoma Res 2010
31123060 MITF-the first 25 years

Arnheiter, H, Goding, CR

Genes Dev 2019
15117313 Rab GTPases and myosin motors in organelle motility

Seabra, MC, Coudrier, E

Traffic 2004
15543135 Rab27A-binding protein Slp2-a is required for peripheral melanosome distribution and elongated cell shape in melanocytes

Fukuda, M, Kuroda, TS

Nat Cell Biol 2004
23652798 Myosin-Va contributes to manifestation of malignant-related properties in melanoma cells

Trindade, DM, Ramão, A, Alves, CP, Yokoyama, S, Pontes, CLS, Sousa, JF, Moraes, MH, Fisher, DE, Espreafico, EM

J Invest Dermatol 2013
21258399 Essential role of microphthalmia transcription factor for DNA replication, mitosis and genomic stability in melanoma

Ye, T, Cormont, M, Keime, C, Kobi, D, Davidson, I, Le Gras, S, Giuliano, S, Bonet, C, Bertolotto, C, Ballotti, R, Strub, T

Oncogene 2011
27939378 MYO5A Gene Is a Target of MITF in Melanocytes

Alves, CP, Yokoyama, S, Sousa, JF, Goedert, L, Fisher, DE, Pontes, CLS, Espreafico, EM

J Invest Dermatol 2017
18281284 Microphthalmia-associated transcription factor regulates RAB27A gene expression and controls melanosome transport

Bertolotto, C, Bille, K, Abbe, P, Beuret, L, Busca, R, Flori, E, Chiaverini, C, Ballotti, R, Bahadoran, P, Ortonne, JP

J Biol Chem 2008
25065759 Myosin-Va and dynamic actin oppose microtubules to drive long-range organelle transport

Robinson, C, Montoliu, L, Sviderskaya, EV, Briggs, DA, Tooth, DJ, Evans, RD, Cantero, M, Ramalho, JS, Hume, AN, Patel, S

Curr Biol 2014
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Authored
Rothfels, K  (2023-12-21)
Reviewed
de la Serna, IL  (2024-05-21)
Arnheiter, H  (2024-02-26)
Steingrímsson, E  (2024-11-05)
Vu, HN  (2024-11-05)
Created
Rothfels, K  (2023-12-18)
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