DNMT3A:DNMT3L in nascent RNA transcript:MeR-PIWIL4:2'-O-methyl-piRNA:SPOCD1:DNMT3A:DNMT3L methylates cytosine in DNA

Stable Identifier
R-HSA-9845308
Type
Reaction [transition]
Species
Homo sapiens
Compartment
nucleoplasm
ReviewStatus
5/5
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DNMT3A:DNMT3L in nascent RNA transcript:MeR-PIWIL4:2'-O-methyl-piRNA:SPOCD1:DNMT3A:DNMT3L methylates cytosine in DNA
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A piRNA bound to PIWIL4 (HIWI2) interacts by base complementarity with a nascent transcript of a transposable element, thus bringing PIWIL4 to the transposable element locus (inferred from mouse homologs in Zoch et al. 2020). PIWI4L binds SPOCD1, which recruits DNMT3A and DNMT3L to methylate the DNA of the transposable element (inferred from mouse homologs). PIWI4L also interacts with the NuRD and BAF chromatin modifying complexes, however the roles of the NuRD and BAF complexes in transposon silencing are not known (inferred from mouse homologs in Zoch et al. 2020). TEX15 is required for piRNA-directed DNA methylation, but does not associate with SPOCD1 and may act upstream or in parallel to SPOCD1 (inferred from mouse homologs in Schöpp et al. 2020).
Literature References
PubMed ID Title Journal Year
24939876 Evolutionally dynamic L1 regulation in embryonic stem cells

Castro-Diaz, N, Ecco, G, Coluccio, A, Kapopoulou, A, Yazdanpanah, B, Friedli, M, Duc, J, Jang, SM, Turelli, P, Trono, D

Genes Dev 2014
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Catalyst Activity

DNA (cytosine-5-)-methyltransferase activity, acting on CpN substrates of nascent LINE-1 RNA transcript:MeR-PIWIL4:2'-O-methyl-piRNA:SPOCD1:DNMT3A:DNMT3L [nucleoplasm]

Physical Entity
nascent LINE-1 RNA transcript:MeR-PIWIL4:2'-O-methyl-piRNA:SPOCD1:DNMT3A:DNMT3L [nucleoplasm] (Homo sapiens)
Activity
DNA (cytosine-5-)-methyltransferase activity, acting on CpN substrates (GO:0051719)
Inferred From
Dnmt3a:Dnmt3l in nascent RNA transcript:MeR-Piwil4:2'-O-methyl-piRNA:Spocd11:Dnmt3a:Dnmt3l methylates cytosine in DNA (Mus musculus)
Authored
May, B  (2023-09-24)
Reviewed
Bourc'his, D  (2024-05-14)
Fernandes, LP  (2024-05-14)
Created
May, B  (2023-10-02)
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