TRIM28 (KAP1) binds KRAB-ZFP in KRAB-ZFP:retroelement chromatin

Stable Identifier
R-HSA-9842850
Type
Reaction [binding]
Species
Homo sapiens
Compartment
nucleoplasm
ReviewStatus
5/5
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TRIM28 (KAP1) binds KRAB-ZFP in KRAB-ZFP:retroelement chromatin
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The TRIM28 dimer (also called KAP1 dimer, Fonti et al. 2019, Stoll et al. 2019) binds a subset of KRAB-ZFPs (Friedman et al. 1996, Moosmann et al. 1996, Stoll et al. 2019, also inferred from mouse ZFP809 (Zfp809 gene)) after the KRAB-ZFPs have bound DNA targets. The RBCC domain of TRIM28 binds the KRAB domain of the KRAB-ZFP (Friedman et al. 1996). The KRAB-ZFP ZNF91 has been shown to bind primate-specific retroelements (SVA_D, SVA_F, L1HS) and recruit TRIM28 (Jacobs et al. 2014). Other KRAB-ZFPs that associate with TRIM28 in 2-hybrid experiments are inferred to have the same activity (Schmitges et al. 2016, Imbeault et al. 2017). About three quarters of TRIM28 binding sites in the genome are located in endogenous retroelements and many binding sites correlate with histone H3 lysine-9 trimethylation and DNA methylation, marks of transcriptional silencing (Turelli et al. 2014). Some KRAB-ZFPs bind TRIM28 but do not repress expression while other KRAB-ZFPs do not bind TRIM28 and repress expression, indicating that other mechanisms of repression may exist (Murphy et al. 2016).
Literature References
PubMed ID Title Journal Year
25274305 An evolutionary arms race between KRAB zinc-finger genes ZNF91/93 and SVA/L1 retrotransposons

Katzman, S, Haeussler, M, Salama, SR, Haussler, D, Paten, B, Nguyen, N, Greenberg, D, Ewing, AD, Jacobs, FM

Nature 2014
10653693 Reconstitution of the KRAB-KAP-1 repressor complex: a model system for defining the molecular anatomy of RING-B box-coiled-coil domain-mediated protein-protein interactions

Begg, GE, Jensen, DE, Speicher, DW, Rauscher, FJ, Friedman, JR, Schultz, DC, Peng, H

J Mol Biol 2000
8769649 KAP-1, a novel corepressor for the highly conserved KRAB repression domain

Neilson, EG, Speicher, DW, Fredericks, WJ, Rauscher FJ, 3rd, Friedman, JR, Jensen, DE, Huang, XP

Genes Dev 1996
9016654 Transcriptional repression by RING finger protein TIF1 beta that interacts with the KRAB repressor domain of KOX1

Bourquin, JP, Schaffner, W, Moosmann, P, Georgiev, O, Le Douarin, B

Nucleic Acids Res 1996
31289231 Structure of KAP1 tripartite motif identifies molecular interfaces required for retroelement silencing

Oda, SI, Chong, ZS, Modis, Y, McLaughlin, SH, Stoll, GA, Yu, M

Proc Natl Acad Sci U S A 2019
27852650 Multiparameter functional diversity of human C2H2 zinc finger proteins

Zhong, G, Yin, Y, Campitelli, LF, Greenblatt, JF, Guo, H, Dai, WF, Schmitges, FW, Radovani, E, Emili, A, Hughes, TR, Taipale, J, Jolma, A, Najafabadi, HS, Barazandeh, M, Kanagalingam, T

Genome Res 2016
28273063 KRAB zinc-finger proteins contribute to the evolution of gene regulatory networks

Trono, D, Helleboid, PY, Imbeault, M

Nature 2017
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Inferred From
Trim28 binds Zfp809 in Zfp809:MoMLV (Moloney murine leukemia virus)
Authored
May, B  (2023-08-26)
Reviewed
Bourc'his, D  (2024-05-14)
Fernandes, LP  (2024-05-14)
Created
May, B  (2023-08-29)
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