L protein acts as a cap 2'-O-methyltransferase to modify RSV mRNAs

Stable Identifier
R-HSA-9834003
Type
Reaction [omitted]
Species
Homo sapiens
Related Species
Human respiratory syncytial virus A
Compartment
cytosol
ReviewStatus
5/5
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L protein acts as a cap 2'-O-methyltransferase to modify RSV mRNAs
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5' capping of respiratory syncytial virus (RSV) mRNAs is catalyzed by the polyribonucleotidyl transferase and methyltransferase domains of the catalytic subunit L of the RNA-dependent RNA polymerase (RdRP) complex (Liuzzi et al. 2005). The so-called cap0, labeled m7GpppG- as all RSV transcripts start with guanidine (Liuzzi et al. 2005), is created first, followed by cap methylation and creation of cap1, labeled m7G(5')pppGm- (Liuzzi et al. 2005, Sutto-Ortiz et al. 2021). Cap methylation is not essential for transcription of RSV mRNAs (Liuzzi et al. 2005). The methyltransferase domain of the L protein is required for 2'-O-methylation and creation of cap1, and the complex of L and P proteins is sufficient for the 2'-O-methylation to occur (Sutto-Ortiz et al. 2021).
Literature References
PubMed ID Title Journal Year
33956914 The methyltransferase domain of the Respiratory Syncytial Virus L protein catalyzes cap N7 and 2'-O-methylation

Canard, B, Roymans, D, Sutto-Ortiz, P, Eléouët, JF, Decombe, A, Tcherniuk, S, Rigaux, P, Ysebaert, N, Decroly, E, Vasseur, JJ, Debart, F, Abeywickrema, P, Noël, M

PLoS Pathog 2021
16189012 Inhibitors of respiratory syncytial virus replication target cotranscriptional mRNA guanylylation by viral RNA-dependent RNA polymerase

Landry, S, Gaudette, Y, Lapeyre, N, Bordeleau, J, Massariol, MJ, Lamarre, L, Dô, F, Cartier, M, Simoneau, B, Dansereau, N, Scouten, E, McCollum, RS, Rancourt, J, Lagace, L, Mason, SW, Lawetz, C, Fazal, G, Liuzzi, M, Whitehead, P, Bolger, G

J Virol 2005
Participants
Input
Output
Participates
as an event of
Catalyst Activity

mRNA (nucleoside-2'-O-)-methyltransferase activity of RSV m7GpppG-mRNAs:RdRP:p-M2-1 [cytosol]

Physical Entity
RSV m7GpppG-mRNAs:RdRP:p-M2-1 [cytosol] (Human respiratory syncytial virus A)
Activity
mRNA (nucleoside-2'-O-)-methyltransferase activity (GO:0004483)
Disease
Authored
Orlic-Milacic, M  (2023-05-05)
Reviewed
Bergeron, HC  (2023-11-03)
Created
Orlic-Milacic, M  (2023-05-05)
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