Reactome | Interaction of E3 with substrate and E2-Ub complex

Interaction of E3 with substrate and E2-Ub complex

Stable Identifier

R-HSA-983157

Type

Reaction [binding]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

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Interaction of E3 with substrate and E2-Ub complex

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Ubiquitin E3 ligases confer specificity to ubiquitination by recognizing target substrates and mediating transfer of ubiquitin from an E2 ubiquitin-conjugating enzyme to substrate (Raymond et al. 2009). E3 ligases includes a large, diverse set of proteins characterized by several defining motifs which include a HECT (homologous to E6-associated C-terminus), RING (Really Interesting New Gene) and U-box domains. The E3 ligases can be multisubunit complexes rather than a single polypeptide. Presently three different kinds of E3 complexes have been described called SCF, APC, and VHL. E3 ligases binds to both substrate and an E2 thioesterified with ubiquitin (E2-Ub).

Literature References

PubMed ID	Title	Journal	Year
16954532	A novel family of membrane-bound E3 ubiquitin ligases Ohmura-Hoshino, M, Goto, E, Matsuki, Y, Aoki, M, Mito, M, Uematsu, M, Hotta, H, Ishido, S	J Biochem	2006
12646216	U-box proteins as a new family of ubiquitin ligases Hatakeyama, S, Nakayama, KI	Biochem Biophys Res Commun	2003
19489725	RING domain E3 ubiquitin ligases Deshaies, RJ, Joazeiro, CA	Annu Rev Biochem	2009
19028597	Maturation of human dendritic cells is accompanied by functional remodelling of the ubiquitin-proteasome system Ebstein, F, Lange, N, Urban, S, Seifert, U, Krüger, E, Kloetzel, PM	Int. J. Biochem. Cell Biol.	2009