Reactome | Proteasomal cleavage of substrate

Proteasomal cleavage of substrate

Stable Identifier

R-HSA-983150

Type

Reaction [omitted]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

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The 26S proteasome complex consists of the 20S catalytic core particle which harbours the proteolytically active sites and the regulatory 19S particle which is responsible for substrate interaction. The 26S proteasomal cleavage generates a vast number (perhaps hundreds) of different peptides, depending on the length and sequence of the substrate protein. Only a small fraction of these peptides (nearly 10%) form the exact length to be presented by class I MHC; most (approximately 70%) are too short to bind. The remaining proteasome products (10-20%) are N-terminally extended precursors that require additional cleavage by cytosolic aminopeptidases for presentation by MHC class I molecules.

Literature References

PubMed ID	Title	Journal	Year
11350924	26S proteasomes and immunoproteasomes produce mainly N-extended versions of an antigenic peptide Rock, KL, Hilton, C, Kisselev, AF, Cascio, P, Goldberg, AL	EMBO J	2001
15224091	Generation of major histocompatibility complex class I antigens: functional interplay between proteasomes and TPPII Kloetzel, PM	Nat Immunol	2004
15224092	Post-proteasomal antigen processing for major histocompatibility complex class I presentation Rock, KL, Goldberg, AL, York, IA	Nat Immunol	2004
9380723	Two distinct proteolytic processes in the generation of a major histocompatibility complex class I-presented peptide Rock, KL, Goldberg, A, Akopian, T, Craiu, A	Proc Natl Acad Sci U S A	1997
15571818	The proteasome and MHC class I antigen processing Kloetzel, PM	Biochim Biophys Acta	2004
14734113	Proteasome and peptidase function in MHC-class-I-mediated antigen presentation Ossendorp, F, Kloetzel, PM	Curr Opin Immunol	2004