MITF-M dimer binds the BCL2 promoter

Stable Identifier
R-HSA-9825756
Type
Reaction [binding]
Species
Homo sapiens
Compartment
nucleoplasm
ReviewStatus
5/5
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MITF-M dimer binds the BCL2 promoter
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MITF binds to an E-box element in the promoter of the BCL2 gene in melanocytes and melanoma cells as assessed by ChIP and EMSA, and upregulates its expression (McGill et al, 2002). BCL2 is an anti-apoptotic factor and consistent with this, overexpression of BCL2 reverses the apoptotic death seen in a dominant negative MITF melanoma cell background (McGill et al, 2002; reviewed in Goding and Arnheiter, 2019; Cheli et al, 2010).
Overexpression of BCL2 in melanoma cells lines reduces miR-211 expression and decreased MITF occupancy at promoters of target genes such as MLANA and TRPM1 (De Luca et al, 2016). A direct interaction was demonstrated between MITF and BCL2 in melanoma cells overexpressing BCL2, but with no change in MITF mRNA or protein levels. Instead, overexpression of BCL2 resulted in a significant decrease in the proportion of MITF localized to the nucleus (De Luca et al, 2016).
Literature References
PubMed ID Title Journal Year
12086670 Bcl2 regulation by the melanocyte master regulator Mitf modulates lineage survival and melanoma cell viability

Golub, TR, Lin, YL, Jackson, IJ, Fisher, DE, Jordan, SA, Ramaswamy, S, Korsmeyer, SJ, Widlund, HR, Ding, HF, McGill, GG, Horstmann, M, Avery, W, Nishimura, EK, Motyckova, G, Du, J

Cell 2002
19995375 Fifteen-year quest for microphthalmia-associated transcription factor target genes

Bertolotto, C, Cheli, Y, Ohanna, M, Ballotti, R

Pigment Cell Melanoma Res 2010
31123060 MITF-the first 25 years

Arnheiter, H, Goding, CR

Genes Dev 2019
26599548 miR-211 and MITF modulation by Bcl-2 protein in melanoma cells

Trisciuoglio, D, Pelosi, A, Del Bufalo, D, Di Martile, M, Candiloro, A, Desideri, M, Farini, V, Bellei, B, Regazzo, G, Tupone, MG, De Luca, T, Rizzo, MG, D'Aguanno, S

Mol Carcinog 2016
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Authored
Rothfels, K  (2023-01-26)
Reviewed
de la Serna, IL  (2024-05-21)
Arnheiter, H  (2024-02-26)
Steingrímsson, E  (2024-11-05)
Vu, HN  (2024-11-05)
Created
Rothfels, K  (2023-01-26)
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