Reactome | MEN1 binds MLL2 complex

MEN1 binds MLL2 complex

Stable Identifier

R-HSA-9818682

Type

Reaction [binding]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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MEN1 (also known as Menin), a tumor suppressor protein mutated in multiple type I endocrine neoplasia, binds to the MLL2 complex. The endogenous complex of MEN1 and MLL2 was co-immunoprecipitated from human cervical carcinoma cell line HeLa and human embryonic kidney cell line HEK293 (Hughes et al. 2004; Jiang et al. 2013: recombinant human KMT2B was used for co-immunoprecipitation from HEK293 cells). The KMT2B (also known as MLL2), similar to the closely related KMT2A (also known as MLL1 or MLL), contains the menin-binding motif (MBM) at its N-terminus, spanning amino acid residues 16–35 (Huang et al. 2012). Based on quantitative studies in HeLa cells, MEN1 has 4- to 5-fold-lower abundance in MLL1/MLL2 complexes than KMT2A/KMT2B, indicating that not all MLL1/2 complexes contain menin (van Nuland et al. 2013).

Huang et al. 2012: recombinant human MEN1 and KMT2B were used.

Literature References

PubMed ID	Title	Journal	Year
23508102	Quantitative dissection and stoichiometry determination of the human SET1/MLL histone methyltransferase complexes Pallaki, P, Smits, AH, van Nuland, R, Jansen, PW, Timmers, HT, Vermeulen, M	Mol Cell Biol	2013
22327296	The same pocket in menin binds both MLL and JUND but has opposite effects on transcription Lei, M, Huang, J, Wan, K, Veniaminova, NA, Hua, X, Matkar, S, Gurung, B, Wan, B, Merchant, JL	Nature	2012
14992727	Menin associates with a trithorax family histone methyltransferase complex and with the hoxc8 locus Hess, JL, Levine, SS, Meyerson, M, Hughes, CM, Bhattacharjee, A, Copeland, TD, Milne, TA, Shanmugam, KS, Kay, GF, Hayes, DN, Rozenblatt-Rosen, O, Biondi, CA, Lee, JC, Hayward, NK	Mol. Cell	2004
23995757	Regulation of transcription by the MLL2 complex and MLL complex-associated AKAP95 Tang, Z, Roeder, RG, Jiang, H, Shimada, M, Dou, Y, Lu, X	Nat Struct Mol Biol	2013