Forkhead box protein A2 (FOXA2, also called HNF3B) is first expressed at the anterior end of the primitive streak and then persists in notochord and definitive endoderm (inferred from the mouse homolog). The FOXA2 gene contains enhancers that activate and enhance expression in the node and notochord (inferred from the mouse homolog). NODAL signaling activates SMAD2, SMAD3 which then interact with FOXH1 at the FOXA2 promoter to activate transcription of FOXA2 (inferred from endoderm in Kim et al. 2011, also inferred from mouse ebryonic stem cells). TEAD proteins also bind the FOXA2 promoter and interact with YAP1 to enhance transcription (inferred from mouse homologs). FOXA2 is required for formation of the node and notochord (inferred from mouse embryos).