SARS-CoV-2 M, N bind to PDPK1

Stable Identifier
Reaction [binding]
Homo sapiens
Related Species
Severe acute respiratory syndrome coronavirus 2
Locations in the PathwayBrowser
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout
SARS-CoV-2 membrane glycoprotein M induced caspase-dependent apoptosis in M-expressing African green monkey kidney epithelial Vero E6 cells and in human liver carcinoma HepG2 cells (Ren Y et al. 2021). The expression of viral M in human embryonic kidney 293T (HEK293T) cells inhibited PKB/AKT signaling pathway and reduced the phosphorylated levels of PKB/AKT, FKHRL1 and ASK. The compromised PKB/AKT activity results in apoptosis via caspase activation. Mechanistically, viral M binds to 3-phosphoinositide-dependent protein kinase 1 (PDPK1 or PDK1), inhibiting its phosphorylation activity on the PH domain. This interection prevented AKT binding to PDPK1 thus down-regulating PDPK1-PKB/AKT signaling (Ren Y et al. 2021). Similar findings were reported for the SARS-CoV-1 M protein (Tsoi H et al. 2014), In addition, SARS-CoV-2 N enhanced M-induced apoptosis by strengthening the interaction between M and PDPK1 (Ren Y et al. 2021).
Literature References
PubMed ID Title Journal Year
34513728 SARS-CoV-2 Membrane Glycoprotein M Triggers Apoptosis With the Assistance of Nucleocapsid Protein N in Cells

Wu, D, Shu, T, Wang, A, Qiu, Y, Huang, M, Zhou, X, Jin, L, Fang, Y, Ren, Y, Zhou, W, Min, J, Wang, C

Front Cell Infect Microbiol 2021
Name Identifier Synonyms
COVID-19 DOID:0080600 2019 Novel Coronavirus (2019-nCoV), Wuhan seafood market pneumonia virus infection, 2019-nCoV infection, Wuhan coronavirus infection
Cite Us!