SARS-CoV-2 M, N bind to PDPK1

Stable Identifier
R-HSA-9755777
Type
Reaction [binding]
Species
Homo sapiens
Related Species
Severe acute respiratory syndrome coronavirus 2
Compartment
plasma membrane
ReviewStatus
5/5
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SARS-CoV-2 M, N bind to PDPK1
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SARS-CoV-2 membrane glycoprotein M induced caspase-dependent apoptosis in M-expressing African green monkey kidney epithelial Vero E6 cells and in human liver carcinoma HepG2 cells (Ren Y et al. 2021). The expression of viral M in human embryonic kidney 293T (HEK293T) cells inhibited PKB/AKT signaling pathway and reduced the phosphorylated levels of PKB/AKT, FKHRL1 and ASK. The compromised PKB/AKT activity results in apoptosis via caspase activation. Mechanistically, viral M binds to 3-phosphoinositide-dependent protein kinase 1 (PDPK1 or PDK1), inhibiting its phosphorylation activity on the PH domain. This interection prevented AKT binding to PDPK1 thus down-regulating PDPK1-PKB/AKT signaling (Ren Y et al. 2021). Similar findings were reported for the SARS-CoV-1 M protein (Tsoi H et al. 2014), In addition, SARS-CoV-2 N enhanced M-induced apoptosis by strengthening the interaction between M and PDPK1 (Ren Y et al. 2021).
Literature References
PubMed ID Title Journal Year
34513728 SARS-CoV-2 Membrane Glycoprotein M Triggers Apoptosis With the Assistance of Nucleocapsid Protein N in Cells

Wu, D, Shu, T, Wang, A, Qiu, Y, Huang, M, Zhou, X, Jin, L, Fang, Y, Ren, Y, Zhou, W, Min, J, Wang, C

Front Cell Infect Microbiol 2021
Participants
Input
Output
Participates
as an event of
Disease
Name Identifier Synonyms
COVID-19 DOID:0080600 2019 Novel Coronavirus (2019-nCoV), Wuhan seafood market pneumonia virus infection, 2019-nCoV infection, Wuhan coronavirus infection
Authored
Shamovsky, V  (2021-10-27)
Reviewed
Messina, F  (2022-02-18)
Created
Shamovsky, V  (2021-10-11)
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