SARS-COV 7a translocates to the late endosome membrane

Stable Identifier
R-HSA-9754554
Type
Reaction [omitted]
Species
Homo sapiens
Related Species
Severe acute respiratory syndrome coronavirus 2
Compartment
endoplasmic reticulum-Golgi intermediate compartment membrane, late endosome membrane
ReviewStatus
5/5
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SARS-COV 7a translocates to the late endosome membrane
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SARS-CoV-2-encoded ORF7a (7a) was shown to localize to the late endosomal compartment, co-localizing with the marker Rab9 upon co-expression of tagged proteins in HeLa cells (Hayn M et al. 2021). Further, the maturation of autophagosomes was assessed by mCherry-GFP-LC3B reporter system and pH-dependent lysotracker upon expression of viral 7a in human embryonic kidney 293T (HEK293T) cells (Hayn M et al. 2021). The effect of 7a on lysosomal-autophagosomal fusion was assessed by colocalization studies in HeLa cells using the lysosomal marker LAMP1 and the autophagosome marker LC3B (Hayn M et al. 2021). The results suggest that SARS-CoV-2 7a blocks autophagic flux in human cells by reducing acidity of lysosome (Hayn M et al. 2021; Koepke L et al. 2021).
Literature References
PubMed ID Title Journal Year
33974846 Systematic functional analysis of SARS-CoV-2 proteins uncovers viral innate immune antagonists and remaining vulnerabilities

Stürzel, CM, Conzelmann, KK, Hunszinger, V, Müller, JA, Münch, J, Christensen, MH, Prelli Bozzo, C, Zech, F, Sparrer, KMJ, Hayn, M, Stenger, S, Aftab, W, Straub, JH, Kirchhoff, F, Hirschenberger, M, Srinivasachar Badarinarayan, S, Klute, S, Nchioua, R, Conzelmann, C, Forne, I, Imhof, A, Sauter, D, Koepke, L, Schmidt, FI

Cell Rep 2021
34281462 Manipulation of autophagy by SARS-CoV-2 proteins

Kirchhoff, F, Hirschenberger, M, Hayn, M, Koepke, L, Sparrer, KM

Autophagy 2021
Participants
Input
Output
Participates
as an event of
Disease
Name Identifier Synonyms
COVID-19 DOID:0080600 2019 Novel Coronavirus (2019-nCoV), Wuhan seafood market pneumonia virus infection, 2019-nCoV infection, Wuhan coronavirus infection
Authored
Shamovsky, V  (2021-10-27)
Reviewed
Messina, F  (2022-02-18)
Created
Shamovsky, V  (2021-09-29)
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