NUDT1 hydrolyzes O6-methyl-dGTP to O6-methyl-dGMP

Stable Identifier
Reaction [transition]
Homo sapiens
O6-methyl-dGTP + H2O = O6-methyl-dGMP + diphosphate + H+
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NUDT1 (MTH1) catalyzes the reaction of O6-methyl-dGTP and water to form O6-methyl-dGMP and PPi (pyrophosphate) (Jemth et al. 2018). Four NUDT1 proteins have been identified, encoded by a single gene with alternative start codons (Oda et al. 1999). The shortest of these, NUDT1 p18, has been shown to be enzymatically active as a monomer associated with a magnesium ion (Mishima et al. 2004; Sakumi et al. 1993; Takagi et al. 2012). The longer isoforms all consist of the p18 polypeptide with aminoterminal extensions and are presumed to be active as well but have not been experimentally characterized. The p18 isoform is predominantly cytosolic (Kang et al. 1995).
Literature References
PubMed ID Title Journal Year
15133035 Structure of human MTH1, a Nudix family hydrolase that selectively degrades oxidized purine nucleoside triphosphates

Mishima, M, Shirakawa, M, Itoh, N, Takahashi, M, Furuichi, M, Iwai, S, Nakabeppu, Y, Yamagata, Y, Sakai, Y, Kamiya, H

J. Biol. Chem. 2004
30304478 MutT homologue 1 (MTH1) catalyzes the hydrolysis of mutagenic O6-methyl-dGTP

Vallin, KSA, Gustafsson, R, Jemth, AS, Homan, E, Almlöf, I, Henriksson, L, Helleday, T, Stenmark, P, Sarno, A, Warpman Berglund, U, Rasti, A, Bräutigam, L

Nucleic Acids Res 2018
22556419 Human MTH3 (NUDT18) Protein Hydrolyzes Oxidized Forms of Guanosine and Deoxyguanosine Diphosphates: COMPARISON WITH MTH1 AND MTH2

Takagi, Y, Ito, R, Sekiguchi, M, Yamagata, Y, Setoyama, D, Kamiya, H

J. Biol. Chem. 2012
10536140 Multi-forms of human MTH1 polypeptides produced by alternative translation initiation and single nucleotide polymorphism

Itoh, R, Nishioka, K, Oda, H, Taketomi, A, Suzuki, T, Maruyama, R, Nakabeppu, Y, Sekiguchi, M, Yakushiji, H

Nucleic Acids Res. 1999
7782328 Intracellular localization of 8-oxo-dGTPase in human cells, with special reference to the role of the enzyme in mitochondria

Iyama, A, Furuichi, M, Fujiwara, T, Nishida, J, Nakabeppu, Y, Kang, D, Sekiguchi, M, Takeshige, K

J. Biol. Chem. 1995
8226881 Cloning and expression of cDNA for a human enzyme that hydrolyzes 8-oxo-dGTP, a mutagenic substrate for DNA synthesis

Tsuzuki, T, Kakuma, T, Furuichi, M, Maki, H, Kawabata, S, Sekiguchi, M, Sakumi, K

J. Biol. Chem. 1993
Catalyst Activity

hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides of NUDT1 [cytosol]

Orthologous Events
Cross References
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