IRF2 or IRF1 binds the GSDMD gene promoter

Stable Identifier
Homo sapiens
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The transcription factor interferon regulatory factor 2 (IRF2) is essential for the transcriptional activation of gasdermin D (GSDMD) in human and mouse cells (Kayagaki N et al. 2019). IRF2 chromatin immunoprecipitation sequencing (ChIP‑seq) analysis and genome‑wide analysis of IRF2‑binding sequences identified a highly conserved IRF2‑binding site within the GSDMD promoter. A luciferase reporter assay using human embryonic kidney HEK293 cells revealed that IRF2 drives GSDMD promoter activation by binding to its consensus site. IRF2 deficiency markedly reduced GSDMD abundance in mouse bone marrow‑derived macrophages (BDMD) and human EA.hy926 endothelial cells. Further, IRF1 was found to regulate GSDMD gene expression in EA.hy926 cells in the absence of IRF2 (Kayagaki N et al. 2019).

Literature References
PubMed ID Title Journal Year
31113851 IRF2 transcriptionally induces GSDMD expression for pyroptosis

Modrusan, Z, Bertram, EM, Reja, R, Mirrashidi, KM, Watanabe, C, O'Rourke, K, Stowe, IB, Lee, BL, Dixit, VM, Kayagaki, N, Zhang, Y, Cho, V, Kummerfeld, S, Goodnow, CC, Roose-Girma, M, Haley, B, Andrews, TD, Morris, LX, Kornfeld, OS

Sci Signal 2019
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