Reactome | GSDME oligomerizes

GSDME oligomerizes

Stable Identifier

R-HSA-9710254

Type

Reaction [binding]

Species

Homo sapiens

Compartment

cytosol, plasma membrane

ReviewStatus

5/5

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The N‑terminal fragment of gasdermin E (GSDME(1-270)) produced by cleavage by caspase-3 (CASP3) or granzyme B (GZMB) targets the plasma membrane (Rogers C et al. 2017; Wang Y et al. 2017). In vitro oligomerization assays showed that GSDME(1-270) formed dimers and high‑molecular weight oligomers when incubated with purified cell membranes from human embryonic kidney 293 (HEK293T) cells (Rogers C et al. 2019). Structural and biochemical studies suggest that the N‑terminal fragments of gasdermins form membrane‑spanning pores allowing release of inflammatory molecules such as interleukin (IL)‑1β, IL‑18, and high‑mobility group box 1 (HMGB1) and eventually causing cell rupture (Shi J et al. 2015; Ding J et al. 2016; Liu X et al. 2016; Feng S et al. 2018; Mulvihill E et al. 2018). Phosphorylation of GSDME at T6 may influence its pore‑forming activity (Rogers C et al. 2019).

Literature References

PubMed ID	Title	Journal	Year
30976076	Gasdermin pores permeabilize mitochondria to augment caspase-3 activation during apoptosis and inflammasome activation Rogers, C, Erkes, DA, Nardone, A, Aplin, AE, Fernandes-Alnemri, T, Alnemri, ES	Nat Commun	2019
27281216	Pore-forming activity and structural autoinhibition of the gasdermin family Ding, J, Wang, K, Liu, W, She, Y, Sun, Q, Shi, J, Sun, H, Wang, DC, Shao, F	Nature	2016