DICER1 cleaves tRNA

Stable Identifier
Reaction [uncertain]
Homo sapiens
Locations in the PathwayBrowser
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

DICER1 cleaves: tRNA Gln CTG in the D-loop, releasing a 19 nt fragment from the 5' end of the tRNA (Cole et al. 2009); tRNA Gly GCC, releasing a 22 nt fragment, tRF-3b (tRF-3027b), from the 3' end of the tRNA (Maute et al. 2013); precursor tRNA Ile TAT, releasing a 21 nt fragment, known as sRNA-Ile or miR-1983, from the 3' end of the tRNA (Hasler et al. 2016, and inferred from mouse homologs). Most tRFs are thought to arise in a DICER1 independent manner, however, as deletion of DICER1 does not significantly alter tRF expression (Kumar et al. 2014, Kuscu and Kumar et al. 2018).

Literature References
PubMed ID Title Journal Year
25270025 Meta-analysis of tRNA derived RNA fragments reveals that they are evolutionarily conserved and associate with AGO proteins to recognize specific RNA targets

Anaya, J, Mudunuri, SB, Dutta, A, Kumar, P

BMC Biol 2014
23297232 tRNA-derived microRNA modulates proliferation and the DNA damage response and is down-regulated in B cell lymphoma

Basso, K, Holmes, A, Califano, A, Dalla-Favera, R, Sumazin, P, Maute, RL, Schneider, C

Proc Natl Acad Sci U S A 2013
27345152 The Lupus Autoantigen La Prevents Mis-channeling of tRNA Fragments into the Human MicroRNA Pathway

Landthaler, M, Jakob, L, Murakawa, Y, Lehmann, G, Hasler, D, Klironomos, F, Meister, G, Rajewsky, N, Grässer, FA

Mol Cell 2016
29844106 tRNA fragments (tRFs) guide Ago to regulate gene expression post-transcriptionally in a Dicer-independent manner

Su, Z, Kuscu, C, Kiran, M, Malik, A, Dutta, A, Kumar, P

RNA 2018
19850906 Filtering of deep sequencing data reveals the existence of abundant Dicer-dependent small RNAs derived from tRNAs

Thatcher, SR, Cole, C, Brown, JW, Hutvagner, G, Green, PJ, Sobala, A, Barton, GJ, Lu, C, Bowman, A

RNA 2009
Catalyst Activity

ribonuclease III activity of DICER1 [cytosol]

Inferred From
Cite Us!