Maturation of spike protein

Stable Identifier
R-HSA-9694548
Type
Pathway
Species
Homo sapiens
Related Species
Severe acute respiratory syndrome coronavirus 2
ReviewStatus
5/5
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Maturation of spike protein
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This COVID-19 pathway has been created by a combination of computational inference from SARS-CoV-1 data (https://reactome.org/documentation/inferred-events) and manual curation, as described in the summation for the overall SARS-CoV-2 infection pathway.

The viral Spike protein of SARS-CoV-1 is subject to N-glycosylation and palmitoylation. The chaperone calnexin exclusively helps with protein folding. The end product is a homotrimer (Nal et al, 2005). In SARS-CoV-2 the Spike glycosylation patterns were extensively characterized, and consist of both N-glycans and O-glycans attached to about twenty amino acids (reviewed by Petrović et al, 2021; Gong et al, 2021; Shajahan et al, 2021). Although there is no reason for the host's glycosylation enzymes behaving differently than with other host or non-host proteins, direct involvement of host enzymes and chaperones with SARS-CoV-2 Spike glycosylation has not been shown. Indirect evidence from inhibition experiments (Reyes et al, 2021; Franco et al, 2022) is confounded by simultaneous inhibition of glycosylation of other proteins like the ACE2 receptor.
Literature References
PubMed ID Title Journal Year
15831954 Differential maturation and subcellular localization of severe acute respiratory syndrome coronavirus surface proteins S, M and E

Nal, B, Chan, C, Kien, F, Siu, L, Tse, J, Chu, K, Kam, J, Staropoli, I, Crescenzo-Chaigne, B, Escriou, N, van der Werf, S, Yuen, KY, Altmeyer, R

J. Gen. Virol. 2005
34782609 The glycosylation in SARS-CoV-2 and its receptor ACE2

Gong, Y, Qin, S, Dai, L, Tian, Z

Signal Transduct Target Ther 2021
  Glucosidase inhibitors suppress SARS-CoV-2 in tissue culture and may potentiate

Reyes, H, Du, YM, Zhou, TL, Xie, X, Shi, PY, Weiss, S, Block, TM

   
34235568 Glycosylation of SARS-CoV-2: structural and functional insights

Shajahan, A, Pepi, LE, Rouhani, DS, Heiss, C, Azadi, P

Anal Bioanal Chem 2021
  UV-4B potently inhibits replication of multiple SARS-CoV-2 strains in clinically relevant human cell lines

Franco, EJ, Warfield, KL, Brown, AN

   
34495539 The Importance of Glycosylation in COVID-19 Infection

Petrović, T, Lauc, G, Trbojević-Akmačić, I

Adv Exp Med Biol 2021
Participants
Events
Participates
as an event of
Event Information
Go Biological Process
viral protein processing (0019082)
Inferred From
Maturation of spike protein (Homo sapiens)
Disease
Name Identifier Synonyms
COVID-19 DOID:0080600 2019 Novel Coronavirus (2019-nCoV), Wuhan seafood market pneumonia virus infection, 2019-nCoV infection, Wuhan coronavirus infection
Cross References
Mondo
Authored
Stephan, R  (2020-07-22)
Reviewed
Acencio, ML  (2020-09-09)
Created
Cook, J  (2020-07-07)
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