nsp13 binds nsp12

Stable Identifier
R-HSA-9694524
Type
Reaction [binding]
Species
Homo sapiens
Related Species
Severe acute respiratory syndrome coronavirus 2
Compartment
cytosol
ReviewStatus
5/5
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nsp13 binds nsp12
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The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout
nsp13, which functions as the viral helicase, is as a part of the SARS coronavirus 2 (SARS-CoV-2) replication-transcription complex (RTC). Two molecules of SARS-CoV-2 nsp13 are found in the RTC complex. N-terminal domains of each nsp13 interact with N-terminal extensions of each copy of nsp8, while nsp13 molecule interacts with the thumb region of nsp12, the viral RNA-dependent RNA polymerase. One nsp13 molecule of SARS-CoV-2 is tightly bound to the RTC, while the other nsp13 molecule is dissociable (Chen et al. 2020). In SARS-CoV-1, nsp13 has been reported to directly interact with nsp12, and nsp12 was shown to increase the helicase activity of nsp13 (von Brunn et al. 2007, Adedeji et al. 2012, Jia et al. 2019).
Literature References
PubMed ID Title Journal Year
33208736 Architecture of a SARS-CoV-2 mini replication and transcription complex

Yan, L, Zhang, Y, Ge, J, Zheng, L, Gao, Y, Wang, T, Jia, Z, Wang, H, Huang, Y, Li, M, Wang, Q, Rao, Z, Lou, Z

Nat Commun 2020
32783916 Structural Basis for Helicase-Polymerase Coupling in the SARS-CoV-2 Replication-Transcription Complex

Chen, J, Malone, B, Llewellyn, E, Grasso, M, Shelton, PMM, Olinares, PDB, Maruthi, K, Eng, ET, Vatandaslar, H, Chait, BT, Kapoor, TM, Darst, SA, Campbell, EA

Cell 2020
33232691 Cryo-EM Structure of an Extended SARS-CoV-2 Replication and Transcription Complex Reveals an Intermediate State in Cap Synthesis

Yan, L, Ge, J, Zheng, L, Zhang, Y, Gao, Y, Wang, T, Huang, Y, Yang, Y, Gao, S, Li, M, Liu, Z, Wang, H, Li, Y, Chen, Y, Guddat, LW, Wang, Q, Rao, Z, Lou, Z

Cell 2021
Participants
Input
Output
Participates
as an event of
Disease
Name Identifier Synonyms
COVID-19 DOID:0080600 2019 Novel Coronavirus (2019-nCoV), Wuhan seafood market pneumonia virus infection, 2019-nCoV infection, Wuhan coronavirus infection
Cross References
Mondo
Authored
Orlic-Milacic, M  (2020-08-12)
Senff-Ribeiro, A  (2020-08-12)
Reviewed
Acencio, ML  (2020-09-09)
Created
Cook, J  (2020-07-07)
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