Assembly of the SARS-CoV-2 Replication-Transcription Complex (RTC)

Stable Identifier
R-HSA-9694271
Type
Pathway
Species
Homo sapiens
Related Species
Severe acute respiratory syndrome coronavirus 2
ReviewStatus
5/5
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Assembly of the SARS-CoV-2 Replication-Transcription Complex (RTC)
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This COVID-19 pathway has been created by a combination of computational inference from SARS-CoV-1 data (https://reactome.org/documentation/inferred-events) and manual curation, as described in the summation for the overall SARS-CoV-2 infection pathway.

In a sequence of ten reactions, mature non-structural proteins (nsp) generated by cleavage of the SARS-CoV-1 pp1a / pp1ab polyproteins are assembled to form the RTC (Fung & Liu 2019; Kirchdoerfer & Ward 2019). Six of these ten steps have been directly studied in SARS-CoV-2: binding of nsp7 to nsp8 (Gao et al. 2020, Li et al. 2020, Konkolova et al. 2020), recruitment of nsp12 (Gao et al. 2020, Hillen et al. 2020, Li et al. 2020, Yin et al. 2020), binding of nsp14 and nsp10 (Li et al. 2020), binding of nsp13 to nsp12 (Chen et al. 2020) formation of the nsp15 hexamer (Kim et al. 2020), and binding of nsp16 to nsp12 (Li et al. 2020, Rosas-Lemus et al. 2020, Viswanathan et al. 2020).
Literature References
PubMed ID Title Journal Year
32511376 The crystal structure of nsp10-nsp16 heterodimer from SARS-CoV-2 in complex with S-adenosylmethionine

Jaroszewski, L, Maltseva, NI, Minasov, G, Wiersum, G, Joachimiak, A, Rosas-Lemus, M, Shuvalova, L, Godzik, A, Jedrzejczak, R, Kim, Y, Endres, M, Inniss, NL, Satchell, KJF, Kiryukhina, O

bioRxiv 2020
32304108 Crystal structure of Nsp15 endoribonuclease NendoU from SARS-CoV-2

Maltseva, NI, Joachimiak, A, Godzik, A, Jedrzejczak, R, Kim, Y, Endres, M, Wilamowski, M, Michalska, K

Protein Sci. 2020
32838362 Virus-Host Interactome and Proteomic Survey Reveal Potential Virulence Factors Influencing SARS-CoV-2 Pathogenesis

Liang, Q, Liu, C, Zhu, Z, Qu, Y, Zhu, T, Zhang, Y, Xiao, Z, Wu, P, Li, J, Yang, X, Yin, Y, Wang, C, Guo, M, Liu, Z, Tian, X, Wang, X, Peng, C

Med (N Y) 2020
32783916 Structural Basis for Helicase-Polymerase Coupling in the SARS-CoV-2 Replication-Transcription Complex

Maruthi, K, Chait, BT, Malone, B, Llewellyn, E, Campbell, EA, Vatandaslar, H, Grasso, M, Shelton, PMM, Olinares, PDB, Kapoor, TM, Eng, ET, Chen, J, Darst, SA

Cell 2020
32277040 Structure of the RNA-dependent RNA polymerase from COVID-19 virus

Lou, Z, Gao, Y, Zhang, B, Zhu, C, Yang, H, Yan, L, Liu, Z, Zheng, L, Wang, T, Hu, T, Xu, W, Yang, X, Wang, Q, Li, J, Liu, F, Rao, Z, Zhang, Y, Huang, Y, Zhao, Y, Cao, L, Zhu, Y, Zhang, L, Ming, Z, Ge, J, Wang, H, Guddat, LW, Hua, T, Sun, Q

Science 2020
31138817 Structure of the SARS-CoV nsp12 polymerase bound to nsp7 and nsp8 co-factors

Kirchdoerfer, RN, Ward, AB

Nat Commun 2019
32709886 Structural basis of RNA cap modification by SARS-CoV-2

Dai, N, Martínez-Sobrido, L, Kovalskyy, D, Chan, SH, Oladunni, F, Qi, S, Park, JG, Misra, A, Gupta, YK, Viswanathan, T, Hromas, RA, Arya, S

Nat Commun 2020
32438371 Structure of replicating SARS-CoV-2 polymerase

Dienemann, C, Tegunov, D, Cramer, P, Farnung, L, Kokic, G, Hillen, HS

Nature 2020
31226023 Human Coronavirus: Host-Pathogen Interaction

Fung, TS, Liu, DX

Annu. Rev. Microbiol. 2019
32358203 Structural basis for inhibition of the RNA-dependent RNA polymerase from SARS-CoV-2 by remdesivir

Jiang, Y, Wang, X, Shen, DD, Su, H, Tao, SC, Xie, YC, Zhang, Y, Gao, M, Jiang, H, Mao, C, Xu, HE, Zhang, S, Shen, J, Zhou, F, Luan, X, Jiang, HW, Yin, W, Zhao, W, Xu, Y, Shen, Q, Tian, G, Chang, S

Science 2020
32535228 Structural analysis of the putative SARS-CoV-2 primase complex

Boura, E, Nencka, R, Klima, M, Konkolova, E

J. Struct. Biol. 2020
Participants
Events
Participates
as an event of
Disease
Name Identifier Synonyms
COVID-19 DOID:0080600 2019 Novel Coronavirus (2019-nCoV), Wuhan seafood market pneumonia virus infection, 2019-nCoV infection, Wuhan coronavirus infection
Authored
Orlic-Milacic, M  (2020-08-12)
Senff-Ribeiro, A  (2020-08-12)
Reviewed
Acencio, ML  (2020-09-09)
Created
Cook, J  (2020-07-07)
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