Nsp3, nsp4, and nsp6 produce replicative organelles

Stable Identifier
R-HSA-9686015
Type
Reaction [omitted]
Species
Homo sapiens
Related Species
Human SARS coronavirus
Compartment
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nsp3 and nsp4 alone caused considerable ER membrane deformation, producing a perinuclear double-walled maze-like body (MLB), and the nsp3–nsp4 interaction was shown to be absolutely necessary for such membrane rearrangement. Further necessary factors are nsp6 and unidentified host factors (Angelini et al 2013, Sakai et al 2017). nsp6 by itself can form Atg5 and LC3II-positive vesicles classically observed in autophagy (Cottam et al, 2014). However, in mouse hepatitis virus (MHV) infections, EDEM1 and OS9 of the ER-associated degradation system have been shown to be necessary co-factors (Reggiori et al, 2010).

Literature References
PubMed ID Title Journal Year
28738245 Two-amino acids change in the nsp4 of SARS coronavirus abolishes viral replication

Sakai, Y, Kawachi, K, Terada, Y, Omori, H, Matsuura, Y, Kamitani, W

Virology 2017
23943763 Severe acute respiratory syndrome coronavirus nonstructural proteins 3, 4, and 6 induce double-membrane vesicles

Angelini, MM, Akhlaghpour, M, Neuman, BW, Buchmeier, MJ

mBio 2013
24991833 Coronavirus NSP6 restricts autophagosome expansion

Cottam, EM, Whelband, MC, Wileman, T

Autophagy 2014
20542253 Coronaviruses Hijack the LC3-I-positive EDEMosomes, ER-derived vesicles exporting short-lived ERAD regulators, for replication

Reggiori, F, Monastyrska, I, Verheije, MH, Calì, T, Ulasli, M, Bianchi, S, Bernasconi, R, de Haan, CA, Molinari, M

Cell Host Microbe 2010
Participants
Participates
Orthologous Events
Disease
Name Identifier Synonyms
severe acute respiratory syndrome DOID:2945 SARS-CoV infection, SARS
Authored
Reviewed
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