nsp14 acts as a 3'-to-5' exonuclease to remove misincorporated nucleotides from nascent RNA

Stable Identifier
R-HSA-9682603
Type
Reaction [transition]
Species
Homo sapiens
Related Species
Human SARS coronavirus
Compartment
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nsp14 acts as 3'-5' exonuclease (Minskaia et al. 2006, Chen et al. 2007) that preferentially excises mismatched nucleotides from double stranded RNA (Minskaia et al. 2006, Bouvet et al. 2012). Binding to nsp10 increases the exonuclease activity of nsp14 (Bouvet et al. 2012, Subissi et al. 2014, Bouvet et al. 2014). nsp14 increases the fidelity of human SARS coronavirus 1 (SARS-CoV-1) replication by the nsp12 RNA-dependent RNA polymerase by 21-fold (Eckerle et al. 2010).

Literature References
PubMed ID Title Journal Year
22635272 RNA 3'-end mismatch excision by the severe acute respiratory syndrome coronavirus nonstructural protein nsp10/nsp14 exoribonuclease complex

Bouvet, M, Imbert, I, Subissi, L, Gluais, L, Canard, B, Decroly, E

Proc. Natl. Acad. Sci. U.S.A. 2012
17927896 Biochemical characterization of exoribonuclease encoded by SARS coronavirus

Chen, P, Jiang, M, Hu, T, Liu, Q, Chen, XS, Guo, D

J. Biochem. Mol. Biol. 2007
20463816 Infidelity of SARS-CoV Nsp14-exonuclease mutant virus replication is revealed by complete genome sequencing

Eckerle, LD, Becker, MM, Halpin, RA, Li, K, Venter, E, Lu, X, Scherbakova, S, Graham, RL, Baric, RS, Stockwell, TB, Spiro, DJ, Denison, MR

PLoS Pathog. 2010
16549795 Discovery of an RNA virus 3'->5' exoribonuclease that is critically involved in coronavirus RNA synthesis

Minskaia, E, Hertzig, T, Gorbalenya, AE, Campanacci, V, Cambillau, C, Canard, B, Ziebuhr, J

Proc. Natl. Acad. Sci. U.S.A. 2006
25074927 Coronavirus Nsp10, a critical co-factor for activation of multiple replicative enzymes

Bouvet, M, Lugari, A, Posthuma, CC, Zevenhoven, JC, Bernard, S, Betzi, S, Imbert, I, Canard, B, Guillemot, JC, L├ęcine, P, Pfefferle, S, Drosten, C, Snijder, EJ, Decroly, E, Morelli, X

J. Biol. Chem. 2014
25197083 One severe acute respiratory syndrome coronavirus protein complex integrates processive RNA polymerase and exonuclease activities

Subissi, L, Posthuma, CC, Collet, A, Zevenhoven-Dobbe, JC, Gorbalenya, AE, Decroly, E, Snijder, EJ, Canard, B, Imbert, I

Proc. Natl. Acad. Sci. U.S.A. 2014
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
3'-5'-exoribonuclease activity of SARS coronavirus gRNA:RTC:nascent RNA minus strand with mismatched nucleotide [double membrane vesicle viral factory outer membrane]
Physical Entity
Activity
Disease
Name Identifier Synonyms
severe acute respiratory syndrome 2945 SARS-CoV infection, SARS
Authored
Reviewed
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