nsp8 generates RNA primers

Stable Identifier
R-HSA-9681651
Type
Reaction [uncertain]
Species
Homo sapiens
Related Species
Human SARS coronavirus
Compartment
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nsp8 functions as an RNA-dependent RNA polymerase (RdRp) that serves as the primase for nsp12, the main RdRp of the SARS coronavirus 1 (SARS-CoV-1) (Imbert et al. 2006), as it is capable of de novo RNA synthesis (te Velthuis et al. 2011). nsp8 synthesizes short oligonucleotides (less than 6 bases long) using genomic RNA as a template. nsp8 requires at least one cytidine residue in the template sequence for its activity. Activity is dependent on manganese ions (Imbert et al. 2006). nsp8 can also extend primers but is 20-fold less efficient than nsp12 (te Velthuis et al. 2011).

Literature References
PubMed ID Title Journal Year
22039154 The SARS-coronavirus nsp7+nsp8 complex is a unique multimeric RNA polymerase capable of both de novo initiation and primer extension

te Velthuis, AJ, van den Worm, SH, Snijder, EJ

Nucleic Acids Res. 2012
17024178 A second, non-canonical RNA-dependent RNA polymerase in SARS coronavirus

Imbert, I, Guillemot, JC, Bourhis, JM, Bussetta, C, Coutard, B, Egloff, MP, Ferron, F, Gorbalenya, AE, Canard, B

EMBO J. 2006
Participants
Participates
Catalyst Activity

RNA-directed 5'-3' RNA polymerase activity of SARS-CoV-1 gRNA:RTC [double membrane vesicle viral factory outer membrane]

Orthologous Events
Disease
Name Identifier Synonyms
severe acute respiratory syndrome DOID:2945 SARS-CoV infection, SARS
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