Gasdermin E (GSDME/DFNA5) cleavage at D270 by caspase‑3 (CASP3) or by granzyme B (GZMB ) liberates the N‑terminal fragment of GSDME (GSDME(1-270)), which moves to the plasma membrane where it strongly binds to inner leaflet lipids such as phosphatidylinositol (4,5)‑bisphosphate (Rogers C et al. 2017; Wang Y et al. 2017). Residues 1–56 of GSDME include 19 hydrophobic ones thought to be involved in membrane targeting and penetration (Rogers C et al. 2017). The N‑terminal fragment of GSDME is thought to induce secondary necrosis/pyroptosis by forming pores in the plasma membrane (Rogers C et al. 2017; Wang Y et al. 2017).