B3GAT3 dimer transfers GlcA to tetrasaccharide linker

Stable Identifier
R-HSA-9638064
Type
Reaction [transition]
Species
Homo sapiens
Compartment
Golgi membrane, Golgi lumen
Synonyms
A glucuronate moiety is the fourth addition to the tetrasaccharide linker
ReviewStatus
5/5
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B3GAT3 dimer transfers GlcA to tetrasaccharide linker
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Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 (B3GAT3) (Ouzzine et al. 2000) transfer a glucuronate (GlcA) residue via a beta1,3-linkage to a terminal galactose. The B3GATs are homodimeric and require manganese as a cofactor (Kakuda et al. 2004, Ouzzine et al. 2000). The tetrasaccharide linker is now complete, ready to accept further hexosamine additions. The type of hexosamine added is critical in determining which glycosaminoglycan (GAG) is formed.
Literature References
PubMed ID Title Journal Year
10842173 Structure/function of the human Ga1beta1,3-glucuronosyltransferase. Dimerization and functional activity are mediated by two crucial cysteine residues

Magdalou, J, Netter, P, Fournel-Gigleux, S, Gulberti, S, Ouzzine, M

J Biol Chem 2000
14993226 Structural basis for acceptor substrate recognition of a human glucuronyltransferase, GlcAT-P, an enzyme critical in the biosynthesis of the carbohydrate epitope HNK-1

Wakatsuki, S, Kato, R, Kajihara, Y, Kawasaki, T, Ishiguro, M, Tagawa, H, Kakuda, S, Oka, S, Shiba, T

J Biol Chem 2004
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Catalyst Activity

galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity of B3GAT3:Mn2+ dimer [Golgi membrane]

Physical Entity
B3GAT3:Mn2+ dimer [Golgi membrane] (Homo sapiens)
Activity
galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity (GO:0015018)
Orthologous Events
B3GAT3 dimer transfers GlcA to tetrasaccharide linker (Bos taurus)
B3GAT3 dimer transfers GlcA to tetrasaccharide linker (Caenorhabditis elegans)
B3GAT3 dimer transfers GlcA to tetrasaccharide linker (Canis familiaris)
B3GAT3 dimer transfers GlcA to tetrasaccharide linker (Drosophila melanogaster)
B3GAT3 dimer transfers GlcA to tetrasaccharide linker (Mus musculus)
B3GAT3 dimer transfers GlcA to tetrasaccharide linker (Sus scrofa)
B3GAT3 dimer transfers GlcA to tetrasaccharide linker (Xenopus tropicalis)
Authored
Jassal, B  (2011-11-01)
Reviewed
D'Eustachio, P  (2012-03-28)
Created
Jassal, B  (2019-02-25)
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